尼可刹米对丙泊酚抑制新生大鼠延髓脑片呼吸放电的拮抗作用  

Antagonistic effects of nikethamide on propofol depressing respiratory discharge of medullary slices in neonatal rats

作　　者：宋晓荣[1] 千智斌[2] 姬明丽[1] 

机构地区：[1]新乡医学院病理生理学教研室,新乡453003 [2]新乡医学院机能学实验室,新乡453003

出　　处：《郑州大学学报（医学版）》2011年第3期388-390,共3页Journal of Zhengzhou University(Medical Sciences)

基　　金：河南省教育厅自然科学研究计划基金资助项目2010B180024;河南省科技厅科技攻关基金资助项目102102310156

摘　　要：目的:观察尼可刹米对丙泊酚抑制新生大鼠延髓脑片呼吸放电的拮抗作用。方法:制作新生大鼠离体延髓脑片标本(完整保留舌下神经根)。取6个稳定记录到呼吸相关节律性放电活动(RRDA)的延髓脑片标本,先用改良的Kreb’s液(MKS)灌流脑片15min(T0),后用含40μmol/L丙泊酚的MKS灌流脑片20min(T1),然后洗脱,待RRDA基本恢复后用含40μmol/L丙泊酚加5mg/L尼可刹米的MKS灌流脑片20min(T2),记录舌下神经根RRDA,分析RRDA吸气时程(TI)、放电幅度积分(IA)和呼吸周期(RC)的变化。结果:3个给药阶段TI、IA和RC相比,差异均有统计学意义(F=22.837、16.926和31.053,P均<0.05)。与T0阶段比较,T1阶段TI缩短,IA降低,RC延长(P<0.05);与T1阶段比较,T2阶段TI、IA升高(P<0.05)。结论:丙泊酚对新生大鼠延髓脑片呼吸放电有抑制作用,尼可刹米能部分拮抗该作用。Aim： To investigate the antagonistic effects of nikethamide on propofol depressing respiratory discharge of medullary slices in neonatal rats.Methods：The neonatal rat medullary slices were prepared and perfused with modified Kreb’s solution（MKS） for 15 min at first（T0）,then the slices were perfused with 40 μmol/L propofol for 20 min （T1）,then 40 μmol/L propofol plus 5 mg/L nikethamide were perfused for 20 min （T2） after washing out.Results：There were significant differences in TI,IA and RC among the 3 phases（F=22.837,16.926,and 31.053,all P0.05）.Compared with T0,propofol decreased TI,IA,and prolonged RC;nikethamide plus propofol increased TI and IA（P0.05）.Conclusion： Propofol has depressant effects on RRDA of medullary slices in neonatal rats,which could be antagonized by nikethamide partly.

关 键 词：丙泊酚 尼可刹米 新生大鼠 延髓脑片 呼吸放电 

分 类 号：R338.2[医药卫生—人体生理学]