大鼠胃缺血后处理模型的建立  

Establishment of gastric ischemic postconditioning model in rats

作　　者：马小波[1] 杜东书[1] 李豫[1] 张建福[1,2] 张咏梅[2] 

机构地区：[1]徐州医学院生理学教研室,徐州221002 [2]徐州医学院神经生物学研究中心,徐州221002

出　　处：《郑州大学学报（医学版）》2011年第3期399-401,共3页Journal of Zhengzhou University(Medical Sciences)

基　　金：国家自然科学基金资助项目30570671;江苏省教育厅科学研究基金资助项目05KJB310134;徐州医学院科研基金资助项目07KJ58

摘　　要：目的:建立并优化大鼠在体胃缺血后处理模型,并初步探讨缺血后处理对胃黏膜损伤的抗氧化机制。方法:78只大鼠分为13组,每组6只。采用单纯胃缺血-再灌注(GI-R)损伤组和假手术组作对照,通过检测胃黏膜损伤指数,观察缺血后处理时短暂的再灌注(R)-缺血(I)持续时间(R和I分别均为10、20、30和40s组)、R-I循环次数(R20s/I20s反复1、3、5和10次组)以及长时间再灌注开始后给予后处理的时间(R为1、2和4min组),对胃黏膜再灌注损伤的影响,并测定胃黏膜组织丙二醛(MDA)含量与超氧化物歧化酶(SOD)活性的变化。结果:假手术组、GI-R组及4个不同持续时间组胃黏膜损伤指数比较,差异有统计学意义(F=16.574,P<0.001);假手术组、GI-R组及4个不同循环次数组胃黏膜损伤指数比较,差异有统计学意义(F=34.815,P<0.001);假手术组、GI-R组及3个不同开始时间组胃黏膜损伤指数比较,差异有统计学意义(F=48.499,P<0.001)。各组大鼠胃黏膜MDA含量(F=27.250、31.450和27.520,P均<0.001)和SOD活性(F=72.662、57.220和50.650,P均<0.001)比较,差异均有统计学意义。结论:成功构建并优化了大鼠胃缺血后处理模型。Aim： To establish and optimize the gastric ischemic postconditioning（GI-postc） model in rats,and preliminarily investigate the antioxidation mechanism of GI-postc.Methods：A total of 78 rats were randomly divided into 13 groups.Sham group and GI-R group were used as control.The experimental groups included four different R and I time groups （10,20,30,and 40 s R/I）,four different cycle groups （1,3,5,and 10 cycles）,and three different beginning time groups（1,2,and 4 min）.Gastric mucosal injury index was detected.The content of malondialdehyde （MDA） and the activity of superoxidase dismutase （SOD） in gastric mucosa were detected.Results： GI-postc could significantly reduce gastric mucosal injury induced by I/R.There were significant differences among sham group,GI-R group,and four different R and I time groups（F=16.574,P0.001）,among sham group,GI-R group,and four different cycle groups（F=34.815,P0.001）,among sham group,GI-R group,and three different beginning time groups （F=48.499,P0.001）.There were significant differences on MDA（F=27.250,31.450,and 27.520,P0.001） and SOD （F=72.662,57.220,and 50.650,P0.001） among these groups.Conclusion：The GI-postc model in rats has been established and optimized successfully.

关 键 词：缺血-再灌注 缺血后处理 胃黏膜 大鼠 

分 类 号：R245.93[医药卫生—针灸推拿学]