蛛网膜下腔神经阻滞麻醉对兔脊髓神经元形态及c-fos蛋白表达的影响  

Effects of spinal nerve block anesthesia on morphology of spinal cord nerve cells and expression of c-fos in rabbits

作　　者：张勇[1] 李霞[1] 甘子明[2] 

机构地区：[1]新疆医科大学第五附属医院麻醉科,乌鲁木齐830011 [2]新疆医科大学基础医学院解剖学教研室,乌鲁木齐830011

出　　处：《郑州大学学报（医学版）》2011年第3期406-409,共4页Journal of Zhengzhou University(Medical Sciences)

摘　　要：目的：观察蛛网膜下腔神经阻滞麻醉对兔脊髓神经细胞的生物学影响。方法：选用健康新西兰大白兔30只,随机分为实验组和对照组,每组15只。基础麻醉后实验组用5g/L布比卡因进行蛛网膜下腔神经阻滞麻醉（腰麻）,对照组用生理盐水代替局麻药。于麻醉后30min灌注取材,HE染色观察2组兔L5~7节段脊髓神经元形态的变化,免疫组化法检测脊髓神经元中c-fos蛋白的表达。结果：与对照组相比,实验组L5~7节段脊髓灰质后角Ⅲ、Ⅳ板层的小圆细胞及前角区板层外侧大多角细胞胞质中均有尼氏体减少、神经元c-fos蛋白阳性细胞数减少[（68.9±1.4）vs（12.3±1.6）,t=60.352,P〈0.001]和脊髓软脊膜分层或断裂现象（P〈0.001）。结论：蛛网膜下腔神经阻滞麻醉后,兔脊髓相应节段的神经细胞功能受到抑制,脊髓软脊膜有分层或断裂现象。Aim：To obsereve the effect of spinal nerve block anesthesia on morphology of spinal cord nerve cells and expression of c-fos in rabbits.Methods：Thity healthy New Zealand white rabbits were randomly divided into experimental and control group,15 in each group.After basal anesthesia,the rabbits in the experimental group were given 5 g/L bupivacaine for subarachnoid nerve block anesthesia（spinal anesthesia for short）,and those in the control group were given normal saline.After 30 minutes,spinal cord samples were prepared.HE staining and immunohistochemistry were used to observe the morphological changes of neurons and the expression of c-fos,respectively.Results：Compared with those of the control group,Nissl bodies reduced and nuclear bias in the horn of lumbosacral spinal cord gray matter Ⅲ,Ⅳ plate layer of small round cells and anterior horn of the lateral lamina mostly Ⅸ horn cells in experimental group.The expression of c-fos protein in the control group was higher than that in the experimental group[（68.9±1.4） vs （12.3±1.6）,t=60.352,P0.001].Spinal fracture soft meninges of experimental group were layered significantly（P0.001）.Conclusion：The nerve block anesthesia after subarachnoid,spinal cord corresponding segment of the nerve cells function is restrained,spinal cord soft present delamination or cracking phenomena.

关 键 词：蛛网膜下腔 麻醉 脊髓神经细胞 C-FOS 兔 

分 类 号：R614[医药卫生—麻醉学]