抗2型登革病毒特异性免疫球蛋白重链可变区基因的扩增与纯化  

Amplification and Purification of the Variable Region Gene in the Heavy Chain of the Specific Immuoglobulin against Type 2 Dengue Virus

作　　者：左丽[1] 刘乐和[2] 郭辉玉[2] 

机构地区：[1]贵阳医学院免疫学教研室,贵阳550004 [2]中山医科大学微生物学教研室

出　　处：《贵阳医学院学报》1999年第4期349-351,共3页Journal of Guiyang Medical College

摘　　要：提取抗２ 型登革病毒单克隆抗体杂交瘤细胞ｍ ＲＮＡ 和总ＤＮＡ，反转录聚合酶链反应（ＲＴ－ ＰＣＲ） 和ＰＣＲ 扩增并纯化特异性免疫球蛋白重链可变区（ＶＨ） 基因。琼脂糖凝胶和聚丙烯胺凝胶电泳观察结果，该ＶＨ 基因约４５０ ｂｐ 。实验证明，上述两种方法均是分离和扩增ＶＨ 基因的有效方法，但提取ＤＮＡ 后进行ＰＣＲ 较提取ｍＲＮＡ 进行ＲＴ－ ＰＣＲ经济、简单。the hybridoma cell for the monoclonal antibody (McAb) against type 2 dengue virus (DV2) were extracted,the variable region gene in the heavy chain (V H gene) were amplified by RT-PCR and PCR,and purified further. The molecular weight of the V H gene was 420 bp with the analysis of agarose and polyacrylamide gel electrophoresis approximately.The experimental result showed that the V H gene could be isolated through the mRNA and DNA of the hybridoma cell and the McAb was taken out by the RT-PCR and PCR further. But the PCR was cheaper and simpler than the RT-PCR.

关 键 词：重链可变区基因 单克隆抗体 登革热病毒 

分 类 号：R512.8[医药卫生—内科学] R394[医药卫生—临床医学]