复方丹参滴丸对2型糖尿病大鼠肾组织HIF-1α及VEGF表达的影响  

The effects of Dan-shen dripping pills on the expressions of HIF-1α and VEGF in renal tissues of type 2 diabetic rats

作　　者：陈频[1] 郑全林[1] 陈文钰[1] 王艳桥[1] 徐向进[1] 

机构地区：[1]南京军区福州总医院内分泌科,350025

出　　处：《中华保健医学杂志》2011年第3期200-203,共4页Chinese Journal of Health Care and Medicine

基　　金：福建省自然科学基金(2008J0104)

摘　　要：目的观察复方丹参滴丸(DSP)防治2型糖尿病大鼠肾脏病变的效果并初步探讨其作用机理。方法采用高糖高脂喂养结合腹腔注射2%链脲佐菌素(STZ,30mg/kg)方法制备2型糖尿病模型大鼠。干预组经口灌服复方丹参滴丸[500mg/(kg·d),共8周]。检测24h尿微量白蛋白(24hUAlb)、空腹血糖(FBG)、空腹胰岛素(FIns)、胰岛素抵抗指数(HOMA-IR)、血脂(TC、TG、LDL-C、HDL-C)、肌酐(SCr)、尿素(BUN)、血清超氧化物歧化酶(SOD)活力、丙二醛(MDA)含量,以及PASM染色观察肾脏组织病理形态学改变,计算细胞外基质(ECM)堆积指数,采用Westernblot检测肾组织缺氧诱导因子-1α(HIF-1α)、血管内皮生长因子(VEGF)表达。结果与糖尿病模型组比较,复方丹参滴丸干预组24hUAlb、FBG、FIns、HOMA-IR、TC、TG、LDL-C、SCr、BUN、MDA均明显降低(P<0.01),SOD活力增高(P<0.01),肾组织HIF-1α、VEGF表达明显降低(P<0.01),而两组HDL-C比较无明显差异。结论复方丹参滴丸对2型糖尿病大鼠肾脏病变具有明显的改善作用,其机制可能与调控HIF-1α/VEGF缺氧反应通路,减轻肾组织细胞氧化应激造成的损害有关。Objective To observe the effects of Dan-shen dripping pills on the renal failure of type 2 diabetic rats and to primarily study its mechanism. Methods The rats of type 2 diabetic model group were fore-administrated with high-carbohydrate-fat diet and then injected with 2%streptozotocin（30 mg/kg）for once. Dan-shen dripping pills solution were administered orally 500 mg/kg·d for 8 weeks to the treated group. 24h urinary albumin excretion,fasting blood glucose,fasting insulin,HOMA-insulin resistance,total cholesterol,triglyceride,low density lipoprotein cholesterol,high density lipoprotein cholesterol,serum creatinine,blood urea nitrogen,superoxide dismutase,and malondialdehyde were detected. The pathological changes of the kidneys by PASM staining were observed and the expressions of HIF-1α and VEGF in renal tissue were detected by Western blot. Results Compared with DM group,the rats of DSP group showed a significant decrease in 24Ualb,FBG,FINS,HOMA-IR,TC,TG,LDL-C,Scr,Bun and MDA（P〈0.01）,SOD was increased（P〈0.01）. There were no difference of HDL-C between DM and DSP group（P〉0.05）. The expressions of HIF-1α and VEGF in renal tissue were markedly increased in DM group versus NC group（P〈0.01）,and there was a significant decrease in expressions of HIF-1α and VEGF in DSP group versus DM group（P〈0.01）. Conclusions The results suggested the Dan-shen dripping pills have effects on renal failure in type 2 diabetic rates, and its mechanism may be related to regulating HIF-1α/VEGF hypoxia response pathway,anti-oxidation,preventing and remeding injuries in renal cells.

关 键 词：糖尿病肾病 缺氧诱导因子-1Α 血管内皮生长因子 复方丹参滴丸 

分 类 号：R587.1[医药卫生—内分泌]