Access微粒子化学发光仪测定TT_3、TT_4的实验评价  

Evaluation of Access in determination of total T_3 and total T_4

作　　者：王志刚[1] 徐恋梅[1] 毕平安 卢嘉波 

机构地区：[1]浙江省嘉兴市第二医院,314000

出　　处：《江西医学检验》1999年第4期214-215,共2页Jiangxi Journal of Medical Laboratory Sciences

摘　　要：目的为了解化学发光技术对TT3、TT4的实验特点。方法以临床最常用的检测项目TT3、TT4的各项实验室评价指标进行测定，并与放射免疫法进行比较，化学发光采用微粒子固相免疫竞争法。结果线性范围TT3：0．52～10．61nmol／L，TT4：18．00～296．48nmol／L，精密度CV值：批内TT3：8．74％，TT4：4．69％，批间TT3：9．78％，TT4：9．15％，平均回收率TT3：96．24％，TT4：103．41％与放射免疫法测定的相关系数r为TT3：0．9070，TT4：0．8753。结论化学发光各技术指标均达到实验要求，且具有操作简单，报告迅速，无需每次定标，更能适合临床的需求。Objctive To understand the characteristics of chemiluminescence immunoassay (CLIA) and to compare it with radioimmunoassay(RIA) in the determination of total T3(TT3) and total T4(TT4). Methods Competitive microparticle solid - phase immunoassay was used for chemiluminescence. Results The linear range TT3: 0. 52 - 10. 61 nmol/L, TT4: 18. 00 - 296. 48 nmol/L. The intra- assay and inter - assay were TT3: 8. 74%, TT4 :4. 69% and TT3: 9. 78%, TT4: 9.15% respectively. The average rates of spiking recovery were TT3 96. 24% and TT4 103. 4%. The coefficient correlation with RIA were TT3 0. 9070 and TT4 0. 8753(P > 0. 05). All technical indices of chemiluminescence fulfilled quality requirements of the test. Conclusion It has advantages of simple operation, rapid report and no need of calibration every time and meeting clinical requirements immediately.

关 键 词：化学发光免疫法 总T3 总T4 Access微粒子 

分 类 号：R446.6[医药卫生—诊断学]