Effects of acupuncture combined with Fluoxetine on behavior and Treg in mice of chronic depression  

针药结合对慢性抑郁症小鼠行为学的改善及Treg的调节作用(英文)

作　　者：洪敏[1,2] 胡燕[1] 郑劼[1] 姜劲峰 丁兆云[1] 禹良艳[1] 王玲玲 

机构地区：[1]南京中医药大学药学院 [2]针药结合重点实验室

出　　处：《World Journal of Acupuncture-Moxibustion》2011年第2期43-48,62,共7页世界针灸杂志（英文版）

基　　金：Supported by Provincial College and University Natural Science Key Basic Research Project of Education Beurou, Jiangsu Province: 08 KJA 360001;Jiangsu Province Qinglan "Qinglan Engineering"

摘　　要：Objective To investigate effects of acupuncture combined with Fluoxetine on behavior and regulatory T cell （Treg） proportion in mice of chronic depression. Methods Sixty Balb/c mice were randomly divided into a normal control group （group A, n=10） and depression model group （n=50）. Fifty Balb/c mice were irritated with a variety of chronic unpredictable moderate stress stimulations for 21 days to prepare the mouse depression model. After successfully modeling, 50 depression model mice were randomly divided into 5 groups, a model group （group B）, a fluoxetine group （group C）, an acupuncture group （group D） and a combined acupuncture and medicine group （group E） and a sham acupuncture group （group F）, 10 mice in each group. The mice in the group C were intragastrically administrated with Fluoxetine once （20 mg/kg）; for the group D, electroacupuncture （EA） was given at ＂Baihui＂ （百会 GV 20） and ＂Shenting＂ （神庭 GV 24） and for the group F, EA was given at non-acupoint of the buttock for 2 min; for the group E, acupuncture combined with Fluoxetine was used with the same acupoints, methods and medicine as those in the group D and C. The treatment was given once each day, for 21 consecutive days. After treatment the open-field behavior （retention period at the central square, horizontal movement,vertical movement, etc.）, sucrose preferences, body mass, food intake and other behaviors were observed, and the proportion of regulatory T cells （CD4^＋ CD2^＋5 Foxp3^＋ Treg cells） was analyzed with flow cytometry. Results Compared with the normal control group, the open-field behavior showed depression-like changes, and humoral consumption, sucrose preferences, body mass and food intake significantly decreased in the mice of the depression model group. After treatment, compared with the group B, the behaviors of the mice in the group E, C and D were improved in varying degrees; compared with other groups, the group E had more obvious effect on vertical mo目的:观察针药结合对慢性抑郁小鼠的行为学影响及对调节性T细胞(Treg)的作用,探讨其免疫作用机制。方法:Balb/c小鼠60只,正常对照组(A组)10只,其余50只以慢性不可预见性中等强度的应激造成小鼠抑郁模型,造模成功后的50只小鼠,再随机分为5组:模型组(B组)、氟西汀组(C组)、针刺组(D组)、针药结合组(E组)、假针刺组(F组),每组10只,其中C组采用氟西汀灌胃1次(20 mg/kg);D组穴取"百会""神庭", F组在臀部非穴位处针刺,均电针2 min;E组,行针刺和药物治疗,针刺穴位、方法及所用药物与D组和C组相同。均每日1次,连续治疗21天。治疗后观察开场行为(中心格停留时间、水平运动、垂直运动等)、糖水偏好、体质量与摄食量等行为学改变,同时以流式细胞仪检测调节性T细胞(CD4+CD2+5 Foxp+3 Treg)百分数量的变化。结果:慢性不可预见性刺激21 d后,与正常对照组相比,造模小鼠开场行为出现抑郁样变化,体液消耗、糖水偏好、体质量与摄食量均明显下降。治疗后与模型组相比,针药结合组、氟西汀组及针刺组对小鼠行为有不同程度的改善作用;而在垂直运动方面,针药结合组较其他组作用更为明显;针药结合对于高表达的Treg细胞有下调作用。结论:针药结合治疗可早期改善小鼠抑郁症行为表现,其作用可能与调节Treg细胞,从而改善抑郁症的免疫抑制状态有关。

关 键 词：DEPRESSION ACUPUNCTURE Acupuncture Medication Combined Regulatory T cell （Treg） 

分 类 号：R965[医药卫生—药理学] R245[医药卫生—药学]