Toxicogenomic investigation of Tetrahymena thermophila exposed to dichlorodiphenyltrichloroethane (DDT),tributyltin (TBT),and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)  

作　　者：CHANG Yue FENG LiFang MIAO Wei 

机构地区：[1]Key Laboratory of Aquatic Biodiversity and Conservation, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China [2]College of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310035, China [3]Graduate University of Chinese Academy of Sciences, Beijing 100049, China

出　　处：《Science China(Life Sciences)》2011年第7期617-625,共9页中国科学（生命科学英文版）

基　　金：supported by the National Natural Science Foundation of China (Grant Nos. 30870356 and 31071993) to Miao Wei;the Knowledge Innovation Program of Chinese Academy of Sciences (Grant No. KSCX2-YW-G-072) to Miao Wei

摘　　要：Dichlorodiphenyltrichloroethane (DDT),tributyltin (TBT),and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) are persistent in the environment and cause continuous toxic effects in humans and aquatic life.Tetrahymena thermophila has the potential for use as a model for research regarding toxicants.In this study,this organism was used to analyze a genome-wide microarray generated from cells exposed to DDT,TBT and TCDD.To accomplish this,genes differentially expressed when treated with each toxicant were identified,after which their functions were categorized using GO enrichment analysis.The results suggested that the responses of T.thermophila were similar to those of multicellular organisms.Additionally,the context likelihood of relatedness method (CLR) was applied to construct a TCDD-relevant network.The T-shaped network obtained could be functionally divided into two subnetworks.The general functions of both subnetworks were related to the epigenetic mechanism of TCDD.Based on analysis of the networks,a model of the TCDD effect on T.thermophila was inferred.Thus,Tetrahymena has the potential to be a good unicellular eukaryotic model for toxic mechanism research at the genome level.Dichlorodiphenyltrichloroethane （DDT）, tributyltin （TBT）, and 2,3,7,8-tetrachlorodibenzo-p-dioxin （TCDD） are persistent in the environment and cause continuous toxic effects in humans and aquatic life. Tetrahymena thermophila has the potential for use as a model for research regarding toxicants. In this study, this organism was used to analyze a genome-wide microarray generated from cells exposed to DDT, TBT and TCDD. To accomplish this, genes differentially expressed when treated with each toxicant were identified, after which their functions were categorized using GO enrichment analysis. The results sug- gested that the responses of T. thermophila were similar to those of multicellular organisms. Additionally, the context likeli- hood of relatedness method （CLR） was applied to construct a TCDD-relevant network. The T-shaped network obtained could be functionally divided into two subnetworks. The general functions of both subnetworks were related to the epigenetic mechanism of TCDD. Based on analysis of the networks, a model of the TCDD effect on T. thermophila was inferred. Thus, Tetrahymena has the potential to be a good unicellular eukaryotic model for toxic mechanism research at the genome level.

关 键 词：microarray enrichment analysis gene interaction network POLLUTANT 

分 类 号：X503.1[环境科学与工程—环境工程] Q959.116[生物学—动物学]