^(125)I-CYP测定人外周淋巴细胞β肾上腺素能受体方法的建立及鉴定  

Establishment and Identification of the Method Measuring Beta-Adrenoceptors in Human Peripheral Lymphocyte Using 125 I-CYP

作　　者：高海鹏[1] 薛全福[1] 林友华[1] 

机构地区：[1]中日友好医院呼吸科,北京100029

出　　处：《标记免疫分析与临床》1999年第4期227-231,共5页Labeled Immunoassays and Clinical Medicine

基　　金：卫生部科学研究基金

摘　　要：用放射配体结合分析技术建立了１２５Ｉ－ ＣＹＰ测定人外周淋巴细胞β肾上腺素能受体（βＡＲ）的方法， 并进行了系列鉴定。用结合饱和实验、动力学实验及竞争抑制实验来鉴定１２５Ｉ－ＣＹＰ测定人外周淋巴细胞βＡＲ 的可靠性及实用性。结果证明：　１２５Ｉ－ ＣＹＰ与βＡＲ 的结合符合配体可饱和， 结合与解离迅速， 结构特异性等特征。由于此方法需血量少， 特异性高， 操作方便， 结果可靠， 且所需设备简单， 可以在临床及科研中推广使用。In order to establish and identify the method measuring β-adrenoceptor (βAR) in human peripheral lymphocyte using 125 I-cyanopindolol ( 125 I-CYP) with radioligand binding assay (RLBA), binding assay, kinetic experiments and competitive inhibition analysis were used to measure binding of βAR to 125 I-CYP and to identify it.Binding assay showed that there was a saturable process with 4406±484 receptors/per cell at maximal occupancy of the sites and a high affinity ( K d=37.23±3.62pmol/L, n=22).Kinetic studies showed that 125 I-CYP binding to βAR was rapid and reversible.Analysis of inhibition of 125 I-CYP binding to βAR possessed high specificity.Conclusion: The method used in the present study possessed the advantages of lesser blood sample, easiness to operate and reliability of result, therefore, it might be further popularized in clinical applications.

关 键 词：外周淋巴细胞 Β肾上腺素能 受体 ^125I-CYP 

分 类 号：R446.6[医药卫生—诊断学]