Determination of tenuifolin in Polygalae Radix from different regions by RF-HPCE  

反向毛细管电泳法测定不同产地远志中细叶远志皂苷的含量(英文)

作　　者：郝增燕[1] 王洁[1] 张一鸣[1] 陈安家[1] 

机构地区：[1]山西医科大学药学院,山西太原030001

出　　处：《Journal of Chinese Pharmaceutical Sciences》2011年第4期342-346,共5页中国药学（英文版）

基　　金：Natural Science Foundation of Shanxi Province (Grant No.200801107-1)

摘　　要：A simple,low-cost and effective reverse flow-high performance capillary electrophoresis（RF-HPCE）method was developed for the separation and determination of tenuifolin in Polygalae Radix.Through optimization of separation conditions, a buffer of 50 mmol/L borax containing methanol（65：35,v/v）was selected for the separation of analytes.Regression equation revealed good linear relationship between peak area and concentration of the analyte in the range of 0.0563-0.9000 mg/mL （r=0.9988）.The recoveries ranged from 99.50%-104.7%.The results indicated that this method is simple,rapid and accurate. It can be used for the quality control of Polygalae Radix.首次建立了简单、经济、有效的反向毛细管电泳分离测定远志药材中细叶远志皂苷含量的方法。通过对分离条件的选择优化,采用含有35%甲醇(v/v)的50mmol/L的硼砂缓冲液使待测成分得到完全分离;细叶远志皂苷在0.0563-0.9000mg/mL浓度范围内呈良好线性关系,r=0.9988,加样回收率在99.50%-104.7%之间。结果表明该方法简便、快速、准确可靠,可作为远志的质量控制方法。

关 键 词：RF-HPCE TENUIFOLIN Polygalae Radix 

分 类 号：R284.2[医药卫生—中药学]