糖尿病肾病患者蛋白氧化损伤与血清基质金属蛋白酶-2的相关性分析  

Relationship between oxidative protein damage and serum matrix metalloproteinase-2 in diabetic ne-phropathy

作　　者：魏岱林[1] 许娟[1] 亓文波[1] 

机构地区：[1]泰安市中心医院内分泌科,山东省泰安271000

出　　处：《中国医师杂志》2011年第6期769-771,775,共4页Journal of Chinese Physician

摘　　要：目的检测糖尿病肾病患者血清蛋白氧化损伤指标的变化，探讨蛋白氧化损伤在糖尿病肾病发病中的可能机制。方法应用改良后的Witko—Sarsat法和2，4-二硝基苯肼法检测血清晚期氧化蛋白产物（AOPP）水平、蛋白质羰基（PCO）含量，应用ELISA及酶谱法分别检测血清基质金属蛋白酶-2（MMP-2）的表达水平和活性。结果DNl组、DN2组AOPP水平明显高于DM组（78．23±19．30VS61．25±12．13、101．59±30．22VS61．25±12．13，F=41．988，P〈0．01），DNl组、DN2组PCO含量亦明显高于DM组（0．84±0．03VS0．66±0．02、1．05±0．05VS0．66±0．02，F=205．763，P〈0．01），AOPP、PCO与血清MMP-2的表达水平（r=0．460，0．480，P〈0．05）和活性（r=0．385，0．560，P〈0．05）均呈正相关，多元逐步回归分析表明AOPP、PCO为血清MMP-2表达水平（P〈0．05，P〈0．01）和活性（P〈0．01，P〈0．05）的主要影响因素。结论推测蛋白氧化损伤通过改变MMP-2的表达和活性，影响肾脏基质代谢。Objective Serum indicators of oxidative protein damage （OPD） were analyzed to explore the effect on renal MMP/TIMP system of OPD in diabetic nephropathy. Methods AOPP levels and PCO levels were measured by modified Witko-Sarsat＇s method and 2, 4-dinitrophenylhydrazine spectrophoto- metric method, expression of serum MMP-2 were determined by ELISA, and MMP-2 activity were detected by zymography . Results AOPP levels of group DN1, group DN2 were higher than those of group DM （ 78. 23 ±19. 30 vs 61. 25 ±12.13 ,101. 59 ± 30. 22 vs 61. 25 ±12.13 , F =41.988,P 〈0.01）. PCOlev- els of group DN1 and group DN2 were higher than those of group DM （0. 84 ±0. 03 vs 0. 66 ±0. 02,1.05 ± 0. 05 vs 0. 66 ± 0. 02, F = 205. 763, P 〈 0. 01 ）. Pearson correlation analysis indicated AOPP and PCO were positively correlated with the expression （ r = 0. 460,0. 480, P 〈 0. 05 ） and activity （ r = 0. 385, 0. 560, P 〈 0. 05 ） of serum MMP-2. Multiple stepwise regression analysis showed that AOPP and PCO were major influential factors of serum MMP-2 expression （ P 〈0. 05, P 〈0. 01 ） and activity（ P 〈0. 01 , P 〈 0. 05 ）. Conclusions OPD might be involved in the imbalance of renal matrix metabolism, which was correlated with the development of diabetic nephropathy.

关 键 词：糖尿病肾病/代谢 DNA损伤 基质金属蛋白酶2/代谢 蛋白水解产物/代谢 

分 类 号：R587.2[医药卫生—内分泌] R692.9[医药卫生—内科学]