棘球蚴囊液对小鼠脾细胞IL-17及Smad2表达的影响  

Influence of Echinococcus granulosus Fluid on Expression of IL-17 and Smad2 of Mouse Spleen Cells

作　　者：金一帮[1,2] 周洋[1,2] 王俊华[1] 吐尔洪江.吐逊 魏晓丽新疆医科大学第一附属医院新疆包虫病基础医学重点实验室 张志[1,2] 张传山新疆医科大学第一附属医院新疆包虫病基础医学重点实验室 李亮新疆医科大学第一附属医院新疆包虫病基础医学重点实验室 林仁勇新疆医科大学第一附属医院新疆包虫病基础医学重点实验室 温浩[1,2] 

机构地区：[1]新疆医科大学第一附属医院新疆包虫病基础医学重点实验室,新疆乌鲁木齐830054 [2]新疆医科大学第一附属医院消化血管外科中心,新疆乌鲁木齐830054

出　　处：《生物技术》2011年第3期43-47,共5页Biotechnology

基　　金：新疆自然科学基金青年基金项目(2010211B19);国家自然科学基金项目(30960342);新疆包虫病基础医学重点实验室开放课题(XJDX0202-2009-03)资助

摘　　要：目的:观察细粒棘球蚴囊液对体外培养BALB/c小鼠脾细胞白介素(IL)-17和Smad2基因表达的影响。方法:制备小鼠脾细胞悬液,接种于48孔培养板中进行培养,以不加任何干预为对照组,囊液处理组为实验组。定时取样提取总RNA,经反转录成cDNA后用实时荧光定量PCR(qRT-PCR)检测IL-17和Smad2基因的表达。结果:小鼠脾细胞IL-17表达在囊液处理6h和12h后升高(1.000±0.207 VS 3.672±0.746和1.000±0.154 VS 5.525±0.843),差异有统计学意义(P<0.05);小鼠脾细胞Smad2表达在培养1h后升高(1.000±0.077 VS 2.069±0.098)在12h后降低(1.000±0.110 VS 0.247±0.011),差异有统计学意义(P<0.05);协同分析发现IL-17基因与Smad2基因的表达变化呈现负相关(P<0.01),相关系数r=-1。结论:细粒棘球蚴囊液对小鼠脾细胞IL-17和Smad2基因的表达具有上调作用,推测其可能与宿主抗棘球蚴感染的机制有关。Objective：To observe the gene expression of interleukin （IL） - 17 and Smad2 in BALB / c mouse spleen cells treated with Echinococcus granulosus fluid （EgF） in vitro. Method：The spleen ceils were separated by network rubbing and randomly divided into experimental group （treated with EgF ） and the control group. Total RNA was extracted at lh, 3h, 6h and 12h, and the mRNA expression of IL - 17 and Smad2 was detected by using RTq - PCR. Result：The qRT - PCR results showed that the expression of IL - 17 displaying a rising trend in the experimental groups compared with the control group, there was significant difference between 6h and 12h （1. 000 ± O. 207VS 3. 672 ± 746 and 1. 000 ± 154 VS 5. 525 ± 843 ,P 〈0.05）, but the expression of Smad2 in mouse spleen cells showed to increase first and then decreased, there was significant difference at lh and 12h（ 1. 000±0.077 VS 2. 069±0.098 and 1. 000 ± 110 VS 0.247 ± 0.011, P 〈 0.05 ）. There was negative correlation between the expression of IL - 17 and Smad2 （ P 〈 0.01 ）, whose correlation Coefficient r =- 1. Conclusion：Echlnococcus granulosus fluid can upregnlate the expression of Smad2 and IL- 17 in mouse spleen ceils in the early stage, suggesting that it may play an important role in resistance to Echinococcus infection.

关 键 词：棘球蚴 囊液 脾细胞 白介素17 SMAD2 

分 类 号：R381[医药卫生—医学寄生虫学]