IL-1β和瘦素在胚胎着床中对ICAM-1和MMP9的影响  

The affection of interleukin-1β and leptin on MMP9 and ICAM-1 in the embryo implantation

作　　者：郝翠芳[1] 郎翠红[2] 

机构地区：[1]山东省烟台毓璜顶医院生殖中心,264000 [2]青岛大学医学院

出　　处：《中国妇幼保健》2011年第19期2995-2998,共4页Maternal and Child Health Care of China

基　　金：烟台市科技发展计划项目〔2006131-8〕

摘　　要：目的:建立体外胚胎和子宫内膜共培养体系,观察不同浓度的IL-1β和瘦素(leptin)对ICAM-1、MMP9的影响,进而探讨IL-1β和leptin对胚胎早期着床的调节作用。方法:采用免疫细胞化学法检测子宫内膜中ICAM-1和MMP9的表达;采用ELISA方法分别检测经IL-1β、leptin和IL-1ra干预共培养模型后培养液中ICAM-1和MMP9的表达。结果:免疫组化结果显示,ICAM-1主要在腺体细胞中表达,基质细胞仅有少量的表达,而MMP9在子宫内膜基质细胞和腺体细胞中均有表达;ELISA结果显示,与对照组相比,ICAM-1分别经10 ng/ml的IL-1β,100 ng/ml的leptin干预后表达上调(P<0.05),而经0.1ng/ml、1ng/ml的IL-1β,1 ng/ml、10 ng/ml的leptin,0.25μg/ml、2.5μg/ml、25μg/ml的IL-1ra干预后表达无明显变化(P>0.05);MMP9分别经1 ng/ml、10 ng/ml的IL-1β,1 ng/ml、10 ng/ml、100 ng/ml的leptin干预后表达上调(P<0.05),而经0.1 ng/ml的IL-1β,0.25μg/ml、2.5μg/ml、25μg/ml的IL-1ra干预后表达无明显变化(P>0.05)。结论:一定量的IL-1β和leptin可以增加共培养模型中ICAM-1和MMP9的表达。Objective：To study the mechanism of interleukin-1β（IL-1β） and leptin on embryo implantation in the early stage by observing the expression of intercellular adhesion molecule-1（ICAM-1） and metalloproteinase 9（MMP-9） intervened by IL-1β or leptin which were closely related to the implantation in the vial model of embryo and endometrium.Methods：The expressions of ICAM-1 and MMP-9 in the endometrium were detected by immunohistochemistry.However,the expressions of ICAM-1 and MMP-9 in the medium of model which have been intervened seperately by IL-1β,leptin or interleukin-1 receptor antagonist（IL-1ra） were detected by ELISA..Results：Immunohistochemistry：ICAM-1 was mainly expressed in the glandular cells,rarely in stromal cells,but MMP-9 was expressed in endometrial stromal cells and glandular cells.The result of ELISA showed that the expression of ICAM-1 in the medium was upregulated after intervened by IL-1β（10 ng/ml） or leptin（100 ng/ml）（P0.05）.But after separately intervened by IL-1β（0.1 ng/ml,1 ng/ml）,leptin（1,10 ng/ml） or IL-1ra（0.25,2.5,25 μg/ml）,they had no significant difference compared with control group（P0.05）.The result of ELISA showed that the expression of MMP-9 in the medium was upregulated after intervened by IL-1β（1,10 ng/ml） or leptin（1,10,100 ng/ml）（P0.05）.But after separately intervened by IL-1β（0.1 ng/ml） or IL-1ra（0.25,2.5,25 μg/ml）,they had no significant difference compared with control group（P0.05）.Conclusion：Certain amounts of IL-1β and leptin can upregulate the expression of ICAM-1 and MMP-9.

关 键 词：白细胞介素-1Β 瘦素 细胞间粘附分子-1 金属蛋白酶9 

分 类 号：R714.12[医药卫生—妇产科学]