人类免疫缺陷病毒1型同性恋感染者与健康人群Th17/Treg平衡状况的比较研究  

The comparative study of T helper cell type 17/regulatory T cell balance in human immunodeficiency virus type 1-infected homosexual patients and healthy donors

作　　者：李丹[1] 贾曼雪[1] 梁华[1] 彭虹[1] 刘东华[1] 赵阳[1] 邵一鸣 

机构地区：[1]中国疾病预防控制中心性病艾滋病预防控制中心传染病预防控制国家重点实验室,北京102206

出　　处：《微生物与感染》2011年第2期65-71,共7页Journal of Microbes and Infections

基　　金："十一五"国家重大科技专项(2008ZX10001-010;2009ZX10004-903);传染病预防控制国家重点实验室课题(2008SKLID101)

摘　　要：通过探讨人类免疫缺陷病毒1型(HIV-1)同性恋感染者外周血中辅助性T细胞17(Th17)与CD4+CD25hiFoxp3+调节性T细胞(Treg)比例及Th17/Treg平衡状态与疾病进展的关系,初步阐明Th17/Treg失衡在HIV发病机制中的作用和意义。选取54例未经抗病毒治疗的HIV感染者,另有32名健康志愿者作为正常对照。分离外周血单核细胞后,利用流式细胞技术检测Th17和Treg水平。结果表明,在HIV感染者外周血中Th17比例明显低于正常对照组(0.68±0.35vs1.42±0.86,P<0.001),Treg比例明显高于正常对照(6.15±2.12vs4.50±0.76,P<0.001),导致HIV感染者中Th17/Treg比例较正常对照显著降低(0.12±0.07vs0.31±0.17,P<0.001)。研究还发现,Th17/CD4比例与CD4+T细胞计数正相关(r=0.371,P<0.05),与病毒载量不相关;Treg/CD4比例与CD4+T细胞计数负相关,与病毒载量正相关(r=-0.402,P<0.05;r=0.447,P<0.001)。此外,Th17/Treg比例与CD4+T细胞计数正相关,与病毒载量负相关(r=0.525,P<0.001;r=-0.318,P<0.05)。结果提示,HIV感染中存在Th17/Treg失衡现象,与疾病进程密切相关,可能在HIV进展中具有重要作用。The aim of this study is to characterize the changes in interleukin 17（IL-17）-secreting T helper cell type 17（Th17） cell frequencies and CD4＋CD25hiFoxp3＋ regulatory T cell（Treg） in a cohort of chronic human immunodeficiency virus type 1（HIV-1）-infected homosexual patients in China,and to clarify the role of a Th17/Treg imbalance in HIV pathogenesis.A total of 54 untreated chronic HIV-infected individuals and 32 healthy donors were recruited in this study.Peripheral blood mononuclear cells were isolated from EDTA-anticoagulated fresh whole blood and stained to characterize Th17/CD4 and Treg/CD4.Our results indicated that the depletion of Th17 cells（0.68±0.35 vs 1.42±0.86,P0.001） was accompanied by an increase in Treg cells（6.15±2.12 vs 4.50±0.76,P0.001）,resulting in a loss of Th17/Treg balance in HIV infection（0.12±0.07 vs 0.31±0.17,P0.001）.Additionally,Th17/CD4 was positively correlated to CD4＋ T cell counts（r=0.371,P0.05）,and Treg/CD4 was inversely correlated to CD4＋ T cell counts and positively correlated to plasma viral load（r=-0.402,P0.05;r=0.447,P0.001）.A positive correlation was observed between Th17/Treg and CD4＋ T cell counts,and Th17/Treg was negatively correlated to viral load（r=0.525,P0.001;r=-0.318,P0.05）.These results indicate that a loss of immune balance during HIV-1 disease progression may have a critical role in HIV-1 infection and further shed new light into understanding the pathogenesis of HIV-1.

关 键 词：辅助性T细胞17 调节性T细胞 T细胞 人类免疫缺陷病毒感染 

分 类 号：R512.91[医药卫生—内科学]