HPLC法测定大枣环磷酸腺苷(c-AMP)的含量  

Determination of cyclic adenosine 3′,5′-monophosphate in fructus jujubae by high performance liquid chromatography

作　　者：郜文[1] 丁兆毅[1] 徐菲[1] 郭建强[1] 

机构地区：[1]首都医科大学产业经营与管理中心,北京100069

出　　处：《首都医科大学学报》2011年第3期375-378,共4页Journal of Capital Medical University

摘　　要：目的建立大枣环磷酸腺苷（cyclic adenosine 3′,5′-monophosphate,c-AMP）含量的测定方法。方法高效液相色谱法（high performance liquid chromatography,HPLC）,采用MGC18色谱柱（4.6 mm×250 mm,5μm）,流动相为0.05 mol/L KH2PO4-Methanol（90：10-10：90）;梯度洗脱,流速1 mL/min,检测波长为254 nm,柱温为30℃。结果 c-AMP在15.6~500.0 ng范围内呈良好的线性关系（r=1.000）,平均回收率为100.35%±1.58%,RSD为1.58%。结论本方法操作简便、灵敏度高、准确性和重现性好,可作为大枣的质量控制方法。Objective To establish the method of determination of cyclic adenosine 3′,5′-monophosphate（cAMP） in fructus jujubae by high performance liquid chromatography（HPLC）.Methods HPLC analysis was carried out using MGC18（4.6 mm×250 mm,5 μm）.The mobile phase was 0.05 mol/L KH2PO4-methanol（90：10-10：90）,Grade-Washing,the flow rate was 1.0 mL/min,Detected wavelength was set at 254 nm,column temperature was 30 ℃.Results The linear range for c-AMP was 15.6~500 ng（r=1.000）,the average recovery was 100.35%±1.58%,RSD was 1.58%.Conclusion The method is rapid and accurate with good reproducibility which is used for the quality control of c-AMP in jujubae.

关 键 词：高效液相色谱法 环磷酸腺苷 大枣 

分 类 号：R917[医药卫生—药物分析学]