长角血蜱感染汉赛巴尔通体后血淋巴超氧化物歧化酶变化的初步研究  

Enzyme activity of SOD(Superoxide Dismutase) in Haemaphysalis longicornis ticks after hemocoelic inoculation with Bartonella henselae

作　　者：李志芳[1,2] 刘起勇[2] 张卫东[1] 宋秀平[2] 栗冬梅[2] 马怀雷[2] 赵帆[2] 孟凤霞[2] 任东升[2] 靳建超[2] 林杰[2] 吴海霞[2] 

机构地区：[1]郑州大学公共卫生学院流行病与卫生统计学教研室,郑州450001 [2]传染病预防控制国家重点实验室,中国疾病预防控制中心传染病预防控制所,北京102206

出　　处：《中国人兽共患病学报》2011年第6期492-494,共3页Chinese Journal of Zoonoses

基　　金：国家自然科学基金项目(No.30600513)资助

摘　　要：目的测定长角血蜱(Haemaphysalis longicornis)感染汉赛巴尔通体(Bartonella henselae)后不同时间段超氧化物歧化酶的活性。方法用Hamilton 10μL微量进液器将汉赛巴尔通体菌液悬浮液(浓度为105cfu/μL),通过假头与盾板间缘凹处注入已部分吸血的未交配的长角血蜱成蜱血腔内,在染毒后1 h,6 h,12 h,24 h及48 h后收集长角血蜱的血淋巴,使用南京建成超氧化物歧化酶(SOD)试剂盒测定其血淋巴中SOD的活性。同时设立生理盐水对照组,对比两组之间及两组内各时间点是否存在差异。结果对收集到的不同时间段的血淋巴测定超氧化物岐化酶的活性后发现,实验组在感染后48 h内,酶活性一直处在平稳的水平;生理盐水对照组SOD酶活性随着时间的延长先升高后又降低,在12 h达到(11.1999±1.3248)U/mL,并且与实验组此时间段的酶活性相比有统计学差异(P=0.036);而在48 h降为最低,与1 h的酶活性相比有统计学差异(P=0.000)。结论长角血蜱受到一定浓度的汉赛巴尔通体攻击后,血淋巴SOD酶活性在一段时间内受到了抑制。The aim of this experiment was to study enzyme activity of superoxide dismutase （SOD） in Haemaphysalis longicornis ticks and its change over time after Bartonella henselae had been inoculated into them. First, five microlitres of B. henselae suspension （10^5 cfu/μL） were injected into the hemocoelic cavity of partially fed virgin female H. longicornis using a 10 μL Hamilton syringe. Then hemolymph samples were collected at 1 h, 6 h, 12 h, 24 h and 48 h after inoculation. Finally, the activity of SOD was determined with the SOD detection kit manufactured by Jiancheng, Nanjing. The method was also re- peated by using saline solution as control. The enzyme activity of SOD remained steady for 48 h in infected group. Whereas the control group, the enzyme activity peaked at 12 h （11. 1 999±1.3 248U/mL） and higher than infected group, there was a difference between the two groups at 12 h （P=0. 036）; then the activity decreased at 48 h, and lower than that at 1 h（P= 0. 000）. The result showed that the enzyme activity of SOD would be inhibited when H. longicornis was attacked by B. henselae in a certain period, which suggests that the B. henselae stress might be a suppression factor with the enzyme activity of SOD over time or concentration.

关 键 词：汉赛巴尔通体 长角血蜱 超氧化物歧化酶 

分 类 号：R376[医药卫生—病原生物学]