尼妥珠单抗对鼻咽癌CNE-2细胞放射敏感性的影响  

Effect of nimotuzumab on radiosensitivity of nasopharyngeal carcinoma CNE-2 cells

作　　者：花永虹[1] 马胜林[2] 付真富[2] 胡巧英[2] 杜灵彬[2] 江皓[1] 

机构地区：[1]浙江中医药大学研究生院,杭州310053 [2]浙江省肿瘤医院放疗科

出　　处：《浙江医学》2011年第6期836-839,共4页Zhejiang Medical Journal

摘　　要：目的 初步探讨尼妥珠单抗对鼻咽癌CNE-2细胞的放射增敏作用.方法 MTT法检测尼妥珠单抗对CEN-2细胞的半数抑制浓度（IC50）,集落形成实验计算细胞存活率,多靶单击模型拟合细胞存活曲线并计算放射增敏比,流式细胞仪检测尼妥珠单抗联合放疗的凋亡率及细胞周期分布.结果 尼妥珠单抗对CNE-2细胞有增殖抑制作用,24h IC50为945μg/ml,0.2×IC50及0.3×IC50剂量下的放射增敏比分别为1.26和1.38,尼妥珠单抗联合放疗组凋亡率、G0/G1期细胞比例较对照组增多（P＜0.05）.结论 尼妥珠单抗联合照射可提高鼻咽癌CNE-2细胞的放射敏感性并阻碍细胞增殖、促进凋亡和干扰细胞周期分布.Objective To investigate radiosensitive effects of nimotuzumab on nasopharyngeal carcinoma cell line CNE-2. Methods The 50% inhibition concerntration （IC50） of nimotuzumab on cell proliferation was measured by MTT. Cell survival was determined by clonogenic assay, single-hit multi-target mode was used to plot survival curve. The cell cycle and apoptosis were measured by flow cytometry. Results Nimotuzumab inhibited proliferation CNE-2 cells, the 24h IC50 was 945 μ g/ml. The radiosensitization enhancement ratios （SERD0） at 0.2 × IC50 and 0.3 × IC50 of nimotuzumab were 1.26 and 1.38 respectively, and the nimotuzumab followed by radiation had a higher apoptosis rate and GO/G1 phase cells （P 〈 0.05）. Conclusion Nimotuzumab can enhance the radiosentivity of nasopharyngeal carcinoma cell line CNE-2, which was possibly associated with inhibiting cell proliferation, inducing apoptosis, and altering cetl phase distribution.

关 键 词：鼻咽癌 尼妥珠单抗 放射敏感性 

分 类 号：R811.5[医药卫生—放射医学]