塔拉豆多糖研究  

STUDY ON POLYSACCHARIDE IN TARA SEED

作　　者：马文秀[1] 罗庆云 尚征贤 李静[2] 

机构地区：[1]南京林业大学化工学院,江苏南京210037 [2]中国科学院广州化学研究所,广东广州510650

出　　处：《林产化学与工业》1999年第4期29-34,共6页Chemistry and Industry of Forest Products

摘　　要：对我国云南５ 个产地的塔拉豆多糖含量测定表明：内胚乳含多糖７９ ％ ～８６ ％ （ 干基计） ，带壳内胚乳含多糖３４ ％ ～４３ ％ （ 干基计） ，对提纯的多糖样品进行了ＧＣ、ＨＰＧＰＣ、ＩＲ 及１３ＣＮＭＲ 分析，结果表明塔拉豆多糖含Ｄ半乳糖、Ｄ葡萄糖、Ｄ甘露糖，其摩尔比为１－００∶０－３３∶３－０３ ，其数均分子量为Ｍｎ ＝ １ ．８２ ×１０４ ，分散度ｄ ＝ １ ．０５ 。ＩＲ、１３ＣＮＭＲ谱图测定推断：塔拉豆多糖主链由β（１ →４） 键连结的Ｄ吡喃型甘露糖单元及少量Ｄ吡喃型葡萄糖单元所组成。Ｄ半乳糖单元可能按（１ →６） 键附于主链中甘露糖单元上。In this paper,the contents of polysaccharides in tara ( Caesalpinia spinosa) seeds from 5 districts in Yunnan Province were determined.Results showed that contents of polysaccharides amounted to 79 %～86 % in endosperms and 34 %～43 % in endosperms with shell respectively (on dry basis). Purified polysaccharide was identified by GC, HPGPC ,IR and 13 C NMR.Results indicated that tara seed polysaccharide composed of D galactose,D glucose and D mannose units in molar ratio 1.00∶0.33∶3.03,with mean molecular weight Mn=1.82×10 4,dispersity d=1.05.By means of IR 13 C NMR,the structure of the polysaccharide was elucidated that it had a main chain mainly consisted of (1→4)linked D mannopyranosyl units with small amount of D glucopyranosyl units,and a side chain possibly consisted of (1→6)linked D galactosyl units attached to D mannose units of the main chain.

关 键 词：塔拉豆 多糖 豆科植物 葡萄甘露聚糖 半乳糖 

分 类 号：Q949.751.9[生物学—植物学] Q946.3