复合人工湿地-池塘养殖生态系统细菌多样性研究  

Bacterial Diversity Investigation on an Integrated System of Constructed Wetland and Pond Aquaculture

作　　者：姚延丹[1,2] 李谷[2] 陶玲[2,3] 李晓莉[2] 张世羊[2] 赵巧玲[2] 林玉良[2] 

机构地区：[1]上海海洋大学水产与生命学院,上海201306 [2]中国水产科学研究院长江水产研究所,湖北荆州434000 [3]中国水产科学研究院淡水渔业研究中心,江苏无锡214081

出　　处：《环境科学与技术》2011年第7期50-55,共6页Environmental Science & Technology

基　　金：现代农业产业技术体系建设专项资助资金(NYCYTX-49);农业部农业科技成果转化资金项目(2008GB23260394);中国水产科学研究院渔业水体净化技术和系统研究重点开放实验室开放基金(FWT-200701);中国水产科学研究院淡水渔业研究中心中央级公益性科研院所基本科研业务费

摘　　要：于2009年8至10月对比调查研究了由人工湿地-池塘养殖复合构成的池塘循环水养殖系统和传统池塘养殖系统中水体理化和生物特征,并利用变性梯度凝胶电泳技术(DGGE)分析了两系统中的细菌多样性。研究结果表明:试验期间各池塘水温和溶氧随月份呈下降趋势,对照塘NH4+-N和TP显著高于循环塘(P<0.05),而循环塘间无显著差异;各池塘细菌丰度和多样性指数均呈季节性变化,对照塘细菌丰度高于循环塘,且波动明显,而多样性指数却低于循环塘,细菌群落结构趋于简单;循环塘的细菌多样性指数逆水流方向呈增加趋势,P2塘较P4受循环水流的调控作用稍大。相关分析表明,细菌多样性指数与DO显著正相关,与NH4+-N、TP负相关。因此,池塘循环水养殖能够提高水体中细菌的生物多样性,相应池塘生态系统更具稳定性。The bacterial diversity investigation in the integrated system of constructed wetland and pond aquaculture was carried out during the period of Aug.～Oct.2009,using analytic method of DGGE（denatured gradient gel electrophoresis）. The findings presented that temperature and DO of the culture ponds changed over the investigation period,and both bacterial density and diversity in the culture pond varied with change of the season;in addition,the levels of NH4^＋and TP in the control pond were significantly higher（p〈0.05）than those in the recirculation ponds.Correlation analysis pertaining to the investigation revealed that bacterial diversity was remarkably and positively correlated with DO,but negatively with NH＋4and TP.It was concluded that the pond-recirculation aquaculture could enhance bacterial diversity in the ponds thus the pond system became more stable ecologically.

关 键 词：池塘循环水养殖系统 细菌多样性 DGGE 生态系统 

分 类 号：X524[环境科学与工程—环境工程]