ELISA法定性试验室内质控方法探讨  

Discussing Interior Quality Control Method of ELISA Qualitative Test

作　　者：丁海明[1] 潘婉仪[1] 

机构地区：[1]广东省中医院,广州510105

出　　处：《现代检验医学杂志》2011年第3期159-161,共3页Journal of Modern Laboratory Medicine

摘　　要：目的探讨ELISA法定性试验室内质控方法。方法以ELISA法检测弱阳性质控品和900份病人血清的HB-sAg，HBsAb，HBeAg，HBeAb和HBeAb，用Levey—Jennings质控图法和半固定范围法分别分析质控数据。结果单独应用Levey-Jennings质控图法时，900份血清中有25份标本阴阳性出现误判。结论在应用ELISA法定性试验室、内质控方法时，结合Levey—Jennings质控图法和半固定范围法，根据实验目的合理选择适合各实验的质控方法。Objective To discuss ELISA qualitative test inside quality control method. Methods Detected HBsAg,HBsAb, HBeAg,HBeAb and HBcAb of weakly positive QC matter and 900 patients serum by ELISA method,analyse QC data with Levey-Jennings and half hold range method. Results 25 patients serum in 900 patients serum appeared false-negative and false-positive alone utilize Levey-Jennings QC map. Conclusion Combine Levey-Jennings QC map and half hold range method when utilize ELISA qualitative test interior quality control method,reasonable choose fit experimental QC method by experimentation purpose.

关 键 词：酶联免疫吸附试验 室内质控 质量控制 

分 类 号：R446.6[医药卫生—诊断学]