光滑爪蟾抗菌肽的分离纯化和对癌细胞的生长抑制作用  

Isolation of skin-antibacterial peptides in Xenopus laevis and its inhibition to cancer cell-SW480

作　　者：侯峰[1] 潘鹏鹏[1] 李吉平[1] 宋博翠[1] 徐静[1] 高宏伟[1] 

机构地区：[1]军事医学科学院军事兽医研究所,吉林长春130062

出　　处：《中国兽医学报》2011年第7期979-982,共4页Chinese Journal of Veterinary Science

基　　金：国家自然基金资助项目(30972197)

摘　　要：通过凝胶过滤层析及高效液相色谱法,从皮肤分泌物中分离出具有抑菌活性的物质,即光滑爪蟾抗菌肽。以大肠癌细胞为体外实验模型,通过细胞形态学、MTS试验,研究光滑爪蟾抗菌肽对SW480细胞的杀伤和生长抑制作用。结果表明,经过分离纯化,得到较纯的活性物质,即光滑爪蟾抗菌肽。通过细胞形态学观察,光滑爪蟾抗菌肽作用组中,细胞的数量明显减少,细胞明显皱缩,成圆形,细胞间隙增大,死细胞增多。MTS试验表明,光滑爪蟾抗菌肽对SW480的生长抑制作用随着浓度的增加而加强。The Xenopus laevis-antimicrobial peptides was separated and purificed by gel filtration chromatography and HPLC. The colorectal cancer SW480 was used as a model in vitro. The cell morphology, MTS were accepted to study the lethal effect and growth inhibiting of Xenopus laevis-antimicrobial peptides to 8W480. It indicated that a species of Xenopus laevis-antimicrobia｜ peptides was got through gel filtration chromatography and HPLC. The number of cells reduced, the shap of the cells become shrinkage round significantly treated by Xenopus laevis-antimicrobial peptides. The depened on the added concentration of the peptides, lethal effect and growth inhibition of the peptides to SW480.

关 键 词：光滑爪蟾 抗菌肽 生长抑制 

分 类 号：S859.796[农业科学—临床兽医学]