豫医无毛小鼠卵巢观察  

Studies on the ovary of female YYHL mice

作　　者：朱奎成[1] 章金涛[1] 王纯耀[1] 杜春燕[1] 邢金山[1] 

机构地区：[1]郑州大学实验动物中心,河南郑州450052

出　　处：《中国兽医学报》2011年第7期1080-1082,1088,共4页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(39970119);河南省教育厅自然科学研究基金资助项目(2007310019)

摘　　要：为了探讨无毛基因的突变对小鼠卵巢的影响,采用病理组织学和流式细胞术研究豫医无毛小鼠卵巢的形态学以及卵巢颗粒细胞周期和凋亡的变化。结果显示6月龄无毛小鼠卵巢颗粒细胞Ap峰和G0/G1期显著高于2月龄无毛小鼠和2、6月龄昆明小鼠。该研究表明,无毛基因的突变诱发了小鼠卵巢颗粒细胞的凋亡,雌性突变小鼠怀孕困难。To explore the effect of a mutation in the hr gene on ovaries of mouse. The study was designed to analyse the structure,apoptosis and cell cycle in YYHL mice by pathohistology and flow cytometry. The accumulation of cells in the G1 phase and a significant apoptosis were present in ovarian granulose cells of YYHL mice at 6 months compared with Y-YHL＇mice at 2 months and from 2 to 6 months of age in wide-type mice. The results showed that the apoptosis of ovarian granulose cells induced by hairless gene may reduce reproductive capability in female YYHL mice.

关 键 词：无毛小鼠 卵巢 病理组织学 流式细胞术 

分 类 号：S852.166[农业科学—基础兽医学]