猪GSTM2基因的多态性及与生产性状相关性分析  

Polymorphism of porcine GSTM2 gene and its correlation analysis withproduction traits

作　　者：王嘉博[1,2] 祝继原[2] 俄广鑫[2] 杨少成[2] 刘娣 

机构地区：[1]东北农业大学动物科学技术学院,哈尔滨150030 [2]黑龙江省农业科学院,哈尔滨150086

出　　处：《东北农业大学学报》2011年第6期23-27,共5页Journal of Northeast Agricultural University

基　　金："十一五"国家科技支撑计划重点项目(2008BADB2B02)

摘　　要：母猪产仔性状是评价猪场效益的重要指标,也是猪育种工作的重点。谷胱甘肽硫转移酶mu亚基(Glutathione S-transferase Mu2,GSTM2)基因是与母猪生殖系统免疫和生长发育有关的基因。研究采用PCR-SSCP的方法分析了GSTM2基因的单核苷酸多态性,并分析了每个多态位点对母猪繁殖性状的影响。结果表明,G2、G5、G7位点对母猪总产仔数和活产仔数影响显著,这三个位点都是AB基因型个体的总产仔数平均数最高,进一步验证了杂合基因型高于纯合基因型性状的理论。这三个位点对断奶窝平均重影响不显著。G2位点对窝平均初生重影响显著。实现了对GSTM2基因多态性的分析,并与母猪产仔性状和初生窝重等生产性状进行了相关性的分析。得到了基因与性状之间的第一手材料。Litter size traits are important indicators to evaluate the benefit of pig farms and they also play a key role in porcine breeding.Glutathione-S-transferase mu subunit（glutathione S-transferase Mu2,GSTM2） gene is related to the immunization of sow reproductive system and its growth.In this study,PCR-SSCP was carried out to analyze the single nucleotide polymorphisms of GSTM2 gene and the effect of each polymorphic locus on the reproductive traits.The results showed that G2,G5 and G7 sites significantly affected the number of birth and alive;AB genotypes in all these three sites were the highest in the average total number of birth,which further verified the theory that the hybrid genotype is better than the homozygous genotype.All these three sites had no significant effect on the average weaning weight.G2 site had significant effect on the average birth weight.GSTM2 polymorphism and its correlation with litter size traits,birth weight and other production traits were analyzed in this study,by which we have got first-had material between genes and traits.

关 键 词：猪 GSTM2基因 SSCP 生产性状 

分 类 号：S828[农业科学—畜牧学]