rSj26-Sj32-Immunogold-IgG-Dipstick法用于急性日本血吸虫病患者的诊断价值  

The diagnostic value of rSj26-Sj32-Immunogold-IgG-Dipstick assay for the patients with acute schistosomiasis japonicum

作　　者：王敏[1] 李文桂[1] 蔡世飞[1] 

机构地区：[1]重庆医科大学附属第一医院传染病寄生虫病研究所,400016

出　　处：《国际检验医学杂志》2011年第10期1030-1032,共3页International Journal of Laboratory Medicine

基　　金：重庆市科委地方病重大专项基金资助项目(2008AB5055;2008AB5008;2008AB5054)

摘　　要：目的探讨rSj26-Sj32-Immunogold-IgG-Dipstick法用于急性日本血吸虫病患者的诊断价值。方法利用纯化的rSj26-Sj32融合蛋白和日本血吸虫成虫抗原(SjAWA)建立Immunogold-IgG-Dipstick法,检测急性日本血吸虫病患者血清,并以华支睾吸虫病、卫氏并殖吸虫病、棘球蚴病、乙型肝炎、肺结核患者和健康人血清作对照。结果 rSj26-Sj32检测急性日本血吸虫病患者血清的敏感性为96.00%,特异性为97.67%;SjAWA与华支睾吸虫病、卫氏并殖吸虫病和泡型棘球蚴病患者血清存在不同程度的交叉反应,而rSj26-Sj32则未见交叉反应。结论纯化的rSj26-Sj32融合蛋白是1种较好的免疫诊断抗原。Objective To study the diagnostic value of rSj26-Sj32-Immunogold-IgG-Dipstick assay for the patients with acute schistosomiasis japonicum. Methods Serums from patients with acute schistosomiasis japonicum were detected by rSj26 Sj32 Im munogold-IgG-Dipstick and schistosoma japonicum adult worm antigen （SjAWA）-Immunogold-IgC-Dipstick, and serums from patients with clonorchiasis sinensis, paragonimiasis westermani,echinococciasis, hepatitis B,lung tuberculosis and from health donors were also detected as control groups. Results The sensitivity and specificity of rSj26 Sj32 Immunogold IgG Dipstick assay in the detection of acute schistosomiasis japonicum were 96.00% and 97.67%. There exist certain cross reaction between SjAWA and paragonimiasis sinensis,clonorchiasis westermani and alveolar echinococcosis but not between rSj26-Sj32 and the other diseases. Conclusion Purified rSj26-Sj32 is one ideal type of immunodiagnostic antigen.

关 键 词：血吸虫病 日本 病毒融蛋白质类 免疫学试验 Dipstick法 

分 类 号：R532.21[医药卫生—内科学]