人参细胞悬浮培养中蔗糖和无机盐对皂苷生产的影响  

Effect of sucrose and inorganic salt on ginsenoside production of Panax ginseng during cell suspension culture

作　　者：高日[1] 朴炫春[1] 于晓坤[1] 金星爱[2] 廉美兰[1] 

机构地区：[1]延边大学长白山生物功能因子省部共建教育部重点实验室,吉林延吉133000 [2]延边大学延边特产研究所,吉林延吉133000

出　　处：《延边大学农学学报》2011年第2期89-93,共5页Agricultural Science Journal of Yanbian University

基　　金：延边大学"211工程"3期建设项目;中韩合作项目

摘　　要：通过研究人参细胞悬浮培养过程中培养基蔗糖浓度、MS浓度、氮浓度及硝态氮与铵态氮比例对细胞生长及皂苷合成的影响,发现在培养基中添加30 g/L蔗糖时,人参细胞合成皂苷能力最强,蔗糖浓度过低(10 g/L)或过高(70 g/L)均不利于细胞干重增加及皂苷积累.MS培养基浓度减半(0.5 MS)或维持原浓度(1.0 MS),总氮浓度为30 mM时,有利于人参细胞皂苷的合成.MS培养基中硝态氮促进皂苷合成,单独使用硝态氮比与铵态氮混用或铵态氮单独使用更有利于皂苷的合成,皂苷生产量达到最高(158.9 mg/L).In order to produce ginsenoside of Panaac ginseng, the effect of sucrose and MS strength, nitrogen, and NOa^＋/NH4^- concentrations on cell growth and ginsenoside production during cell suspension culture were investigated. The results showed that MS medium supplemented with 30 g/L sucrose promoted production of ginsenoside, low and high sucrose concentrations were not suitable for cell growth and ginsenoside productivity. Production of ginsenode was favorable in 1/2 MS or MS medium containing 30 mM nitrogen. The present of NOa^＋ in MS medium was more important than NH4^- for increment of cell dry weight and accumulation of ginsenoside, 158.9 mg/L of ginsenoside productivity was obtained when MS medium supplemented with 30 mM of sole NO3^＋.

关 键 词：人参细胞 蔗糖 MS浓度 氮 

分 类 号：S567.51[农业科学—中草药栽培]