急性髓性白血病中hMLH1基因启动子区甲基化与表达水平检测及意义  

Promoter methylation status and mRNA transcription of hMLH1 gene in acute myeloid leukaemia and their clinical significance

作　　者：邹菁[1] 陈智超[1] 邹萍[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院血液科,湖北武汉430022

出　　处：《实用医院临床杂志》2011年第4期11-13,共3页Practical Journal of Clinical Medicine

摘　　要：目的检测急性髓性白血病(acute myeloid leukaemia,AML)细胞hMLH1基因启动子甲基化状态及转录水平,探讨其与AML发生发展的关系。方法运用甲基特异性PCR和Real-Time PCR法检测62例AML患者外周血有核细胞中hM-LH1基因启动子甲基化状态及其转录水平,分析白血病细胞hMLH1基因启动子区甲基化与临床资料的关系。结果 62例急性髓性白血病hMLH1基因启动子甲基化频率为14.52%(9/62),甲基化白血病细胞与非甲基化白血病细胞hMLH1 mRNA的相对含量分别是0.51±0.19和0.87±0.24,两者的hMLH1转录水平有明显差异(P<0.05);AML细胞中hMLH1基因启动子甲基化与患者性别及FAB分型无明显相关性,与发病年龄及难治/复发显著相关。结论在AML中hMLH1基因转录水平受基因启动子甲基化调控,且hMLH1基因甲基化差异与白血病发生及进展恶化显著相关。Objective To investigate the promoter methylation status and mRNA transcription in acute myeloid leukaemia（AML）,and the role of them in oncogenesis and progression.Methods Methylation specific PCR（MSP） and real-time PCR were used to detect the methylation status and mRNA expression of hMLH1 gene in peripheral blood cells of acute myeloid leukaemia.The relationship between hMLH1 gene promotor methylation and clinical data was analyzed.Result The methylation frequency of hMLH1gene in AML was 14.52%（9/62）.The relative level of mRNA expression was 0.51±0.19 in 9 methylated AML patients,and 0.87±0.24 in 53 unmethylated AML patients,showing a significant difference between this two statuses（P0.05）.the sexuality and FAB typing were not significant correlated with hMLH1 promoter methylation status,but the age of onset and cancer progression were statistically correlated.Conlusion The transcription of hMLH1 gene was maily regulated by the promotor methylation status in AML,which was also related to the oncogenesis and progression of AML.

关 键 词：急性髓性白血病 HMLH1基因 启动子 甲基化 

分 类 号：R733.71[医药卫生—肿瘤] R34[医药卫生—临床医学]