高效液相色谱法测定胃苏颗粒中橙皮苷和柚皮苷含量  

Determination of Hesperidin and Naringin in Stomach Su Keli by HPLC

作　　者：李沁媛[1] 唐亚琴[1] 徐辉[1] 李艳[1] 边清泉[1] 

机构地区：[1]绵阳师范学院天然产物研究所,四川绵阳621000

出　　处：《西南科技大学学报》2011年第2期82-84,共3页Journal of Southwest University of Science and Technology

基　　金：绵阳市科技局自然科学研究重点项目(10S002-2);四川省教育厅自然科学研究项目(09ZC019);绵阳师范学院科学研究项目(hx2010-2011-08)

摘　　要：目的:用高效液相色谱法测定胃苏颗粒中橙皮苷和柚皮苷的含量。方法:采用DiamonsilTMC18(4.6 mm×150mm,5μm)色谱柱;乙腈-磷酸水(pH=3.5)(22:78,V/)为流动相,流速为1.2 mL/min;.检测波长为283 nm。结果:待测组分与其他组分分离度良好(R>1.5),橙皮苷和柚皮苷线性范围分别为0.25～1.49μg和0.093～0.56μg,橙皮苷和柚皮苷的平均回收率分别为99.2%,98.8%,RSD分别为1.6%,2.3%。结论:高效液相色谱法是测定胃苏颗粒中橙皮苷和柚皮苷成分的一种简便、有效的方法。The content of hesperidin and naringin in Stomach Su Keli was determinated by HPLC. Column is DiamonsilTMcls（4.6 mm × 150 mm, 5 μm）. The mobile phrase is CHzCN- pH 3.5 H3PO4 = 22 ： 78（V/V）. The velocity of flow is 1.20 mL·min-1 and the detective wavelength is 283 nm. The experimental results show that the separate degree of the undeterminated components and others is favourable （R 〉 1.5 ）, the linearity tangs of the content of hesperidin and naringin are from 0.25 to 1.49 μg ·mL^-1 and 0.093 to 0.56 μg·mL^-1, respectively. The average recoveries of hesperidin and naringin are 99.2% and 98.8% and theirRSDs are 1.6% and 2.3%, respectively. HPLC is a convenient and effective method to determine the content of hesperidin and naringin in Stomach Su Keli.

关 键 词：高效液相色谱法 胃苏颗粒 橙皮苷 柚皮苷 

分 类 号：O657.72[理学—分析化学] R917[理学—化学]