感染PRRSV Nsp2基因部分缺失变异株的仔猪抗体变化规律  

The Changed Rule of Infected Piglets by a Mutant of Porcine Reproductive and Respiratory Syndrome Virus with Nsp2 Gene Deletion

作　　者：张凤华[1] 卢晓艳[1] 徐红运[1] 赵琳[1] 刘玉松[1] 范旭[1] 夏平安[1] 崔保安[1] 

机构地区：[1]河南农业大学牧医工程学院,河南郑州450002

出　　处：《华北农学报》2011年第3期68-71,共4页Acta Agriculturae Boreali-Sinica

基　　金：河南省重点攻关项目(92102110018)

摘　　要：为了解PRRSV Nsp2基因缺失变异株的致病性,采用2个Nsp2基因分别连续缺失3个和89个碱基的PRRSV变异株Hn-2(GenBank:FJ237419)和Hn-4(GenBank:FJ237421)的病毒液,人工滴鼻感染断奶仔猪,同时用Marc-145健康仔猪细胞培养液滴鼻作对照,在感染后0,7,14,24,32,41,50,60和70 d无菌采血,分离血清,用RT-PCR方法检测病毒、用N-ELISA方法和免疫荧光抑制试验分别检测抗PRRSV N蛋白抗体和抗PRRSV中和抗体,比较分析不同时期病毒复制和抗体产生的变化规律。结果表明,仔猪感染Hn-2和Hn-4毒株后7～14 d均能检测到病毒,第7天能检测到抗PRRSV N蛋白抗体,并一直维持较高水平,第41和50天检测到较低水平的中和抗体,随后中和抗体效价逐渐升高。试验猪均未出现死亡,表明PRRSV Nsp2基因缺失变异株不一定导致感染猪的死亡。In order to understand the pathogenicity of PRRSV about Nsp2 gene deletion mutant,The PRRS negative healthy piglets of 20 days-old were inoculated by intranasal with a Mutant of PRRSV Hn-2（GenBank：FJ237419）and Hn-4（GenBank：FJ237421）strains respectively,which delete 3 base pairs and 89 base pairs in the Nsp2 gene respectively,while healthy piglets were used for control,the sera were separated aseptically on the 0,7,14,24,32,41,50,60,70 d respectively,using RT-PCR to detect the virus,using ELISA method and immunofluorescence inhibition test for detection of anti-PRRSV N protein antibody and neutralizing antibodies,variation of the virus and antibody were comparied in different periods of time.The results showed that the PRRSV could be detected in 7-14 d after infected with the Mutant Hn-2,and Hn-4 strain,anti-PRRSV N protein antibodies could be detected on the 7th day and has maintained a high level,respectively,the low level of neutralizing antibodies could be detected respectively on 41 d and 50 d,and the neutralization antibody increased gradually.The test pigs were not of death,indicating that the mutant PRRSV with deleted Nsp2 gene does not necessarily lead to pig death.

关 键 词：猪繁殖与呼吸综合征病毒 N蛋白 中和抗体 NSP2基因 

分 类 号：S852.65[农业科学—基础兽医学] Q78[农业科学—兽医学]