湖南湖北两省H_6亚型禽流感病毒表面基因的序列分析  

Surface Genes Analysis of H_6 Subtype Avian Influenza Virus Isolated from Hunan and Hubei Provinces

作　　者：丁晴微[1,2] 于杨[1] 崔鹏飞[1] 齐瑛琳[1] 李印[1] 施建忠[1] 崔佳莹[1] 邱伯根[2] 陈化兰[1] 邓国华[1] 

机构地区：[1]中国农业科学院哈尔滨兽医研究所农业部动物流感重点开放实验室兽医生物技术国家重点实验室,哈尔滨150001 [2]湖南农业大学动物医学院,长沙410128

出　　处：《基因组学与应用生物学》2011年第3期282-287,共6页Genomics and Applied Biology

基　　金：国家重点实验室国家开放基金(SKLVBP201003)资助

摘　　要：2008年至2009年间,在湖南和湖北两省的活禽市场中分离到了14株H6亚型禽流感病毒,为了解这14株病毒之间的分子特征和差异,我们运用PCR和测序鉴定对这14株病毒的NA基因进行了分型,并对其表面基因HA和NA进行序列测定及序列分析。14株H6亚型病毒中,H6N2亚型12株,H6N6亚型2株。序列测定和进化分析结果显示:DK/HN/284的HA基因与其它13株的HA差异性较大,差异性达到19.4%～20.2%,其余13株毒同源性在94.2%～99.9%;N2亚型NA基因的同源性在91.1%～99.9%,差异性比较大;两株N6亚型NA基因同源性为89.5%,差异明显。这些数据表明:不同毒株呈现一定的地域性差异。与我国周边其它地区的H6亚型禽流感毒株序列进行比较发现,只有DK/HN/284的HA基因与香港早期的毒株可能有着共同的来源,其余都与香港和韩国等的毒株有着较大的差异性,并且各个毒株的HA基因上潜在的糖基化位点和受体结合位点也有所不同,这些数据表明,这些毒株表现出明显的异源性。14 H6 subtype AIVs were isolated in the living birds markets in Hunan and Hubei provinces between 2008 and 2009.In order to understand the molecular characteristics and differences of the 14 virus strains,we maded gene classification by PCR and sequenced and analysised the HA and NA genes.There were 12 strains of viruses belonged to H6N2 subtype and 2 strains belong to H6N6 subtype.The HA,NA sequence analysis proved that 13 HA sequences homology were quite high which reached 94.2%～99.9% while the differences in HA of DK/HN/284/09 which reached 19.4%～20.2% compared to other 13 H6 AIVs.The NA genes homology were 91.1%～99.9% among 12 H6N2 AIVs and there were much differences in N6 NA gene whose homology was only 89.5%.All these data indicated the emergence of different strains on a kind of geographical difference.There was only DK/HN/284/09 HA gene which had relative high homology compare with other H6 subtype AIVs isolated from neighboring countries or areas.Most of viruses HA gene had different potential glycosylation sites and receptor binding sites,which showed significant heterogeneity.

关 键 词：禽流感病毒 H6亚型 HA NA 基因分析 

分 类 号：S852.65[农业科学—基础兽医学]