两株香蕉枯萎病拮抗菌在香蕉体内的定殖  

The Colonization of Two Strains of Antagonistic Bacteria of Fusarium oxysporum in Banana

作　　者：汪腾[1,2] 段雅婕[1,2] 刘兵团[2,3] 郭刚[4] 周登博[2] 谭昕[2] 张锡炎[2] 黄绵佳[1] 

机构地区：[1]海南大学园艺园林学院,海口571737 [2]中国热带农业科学院热带生物技术研究所,海口571101 [3]湖南农业大学生物科学技术学院,长沙410128 [4]中国热带农业科学院海口实验站/香蕉研究所,海口570102

出　　处：《基因组学与应用生物学》2011年第3期342-350,共9页Genomics and Applied Biology

基　　金：公益性行业科技"香蕉现代产业技术体系研究与建立"(Nyhyzx07-029);"国家香蕉产业体系建设"(Nycytx-33)共同资助

摘　　要：本研究将2株香蕉枯萎病拮抗细菌0202和1112分别涂布于含有不同浓度的利福平培养基上,筛选出抗药性标记菌株0202-r和1112-r。将香蕉枯萎病菌Foc4接种于香蕉植株根部,3d后再将抗药性标记菌株接种在同一部位或位点,于不同时间测定拮抗细菌在根际、根内、球茎和假茎的定殖情况。研究结果显示,将拮抗细菌接种香蕉根部1～3d后,在植株根际土壤内存在大量拮抗细菌,而根内、球茎和假茎内存在少量拮抗细菌;接种7～14d后,在植株根内、球茎和假茎内定殖的拮抗细菌增多,并达到最大量;在接种14～21d后,在植株根内、球茎和假茎内定殖的拮抗细菌量急剧下降;在接种28~35d后,在植株根内、球茎和假茎内定殖的拮抗细菌量稍有回升。Two strains of Fusarium oxysporum f.sp.cubense antagonistic bacteria 0202 and 1112,were spreaded on medium containing different concentrations of rifampicin respectively.Two rifampilin-resistant strains 0202-r and 112-r were selected.We inoculated the Foc4 to the banana plants roots.3 days later,we inoculated the antagonistic bacteria at the same position or sites.We measured the colonization situation of antagonistic bacteria in the rhizosphere,roots,bulbs and pseudostem at different time.The results showed that when the antagonistic bacteria were inoculated to banana roots after 1～3 days,there were a large number of bacteria existed in the rhizosphere soil of plants,while a small amount of antagonistic bacteria existed in roots,bulbs and pseudostem;after 7～14 days,the No.of antagonistic bacteria colonization in roots,corm and pseudostem increased,even reached the maximum amount;after 14～21 days,the No.of antagonistic bacteria colonization inplant roots,bulbs and planting false stems declined sharply;after 28～35 days,the No.of antagonistic bacteria colonization inplant roots,bulbs and planting false stems slight increase.

关 键 词：香蕉枯萎病 拮抗细菌 抗生素标记 定殖力 

分 类 号：S436.68[农业科学—农业昆虫与害虫防治]