雌激素对大鼠脑创伤后海马区氧应激状态及bax表达的影响  

Estrogen attenuates neuron apoptosis by inhibiting ROS and bax expression following traumatic brain injury in rat

作　　者：田艳霞[1] 江小华[1] 刘桦[1] 卢鹤翔[1] 赵彬[1] 崔建忠[2] 高俊玲[1] 

机构地区：[1]华北煤炭医学院组胚教研室,河北唐山063000 [2]唐山市工人医院

出　　处：《中国实验诊断学》2011年第6期951-953,共3页Chinese Journal of Laboratory Diagnosis

基　　金：河北省自然科学基金资助项目(C2009001247);中国人事部留学归国基金(2007-17)

摘　　要：目的探讨雌激素在颅脑创伤中发挥保护作用的分子机制。方法按照Marmarou's方法建立大鼠创伤性脑损伤(TBI)模型。100只雄性SD大鼠随机分为创伤组、治疗组和对照组,其中创伤组和治疗组又分为伤后10 min、30min、3 h、6 h、24 h、48 h和72 h 7个时相组。采用硫代巴比妥酸法测定氧自由基中间产物丙二醛(MDA),免疫组化方法检测细胞凋亡相关基因bax的表达,原位缺口末端标记法(TUNEL)检测凋亡细胞。结果雌激素治疗组海马区MDA、bax的表达及TUNEL阳性细胞数均明显低于模型组。结论大鼠脑创伤后,雌激素通过抗氧化作用抑制凋亡基因bax的表达,进而抑制神经细胞凋亡而起到脑保护作用。Objective To investigate the mechanism of estrogen＇s protection effect on neurons in traumatic brain injury（TBI） in rats.Methods The rat model of TBI was made according to the method of Marmarou＇s（1994）.Male Sprague-Dawley rats were randomly divided into control,TBI and estrogen treatment groups.The rats in TBI and treatment groups respectively were divided into 7 subgroups according to the time phase after brain injury,which were 10 min,30 min,3 h,6 h,24 h,48 h and 72 h.The value of MDA was measured by thio-barbiturie acid.Theexpression of bax was detected by immunohistochemistry and the apoptosis of neurons wasdetected by TUNEL method.Results The value of MDA,immuno-activity of bax and thenumber of TUNEL positive cells of treatment group were lower than TBI group.Conclusion Estrogen attenuates the apoptosis of neurons by reducing oxygen free radical and then bax expression following traumatic brain injury in rats.

关 键 词：大鼠 雌激素 氧自由基 海马 颅脑创伤 BAX 凋亡 

分 类 号：R651.1[医药卫生—外科学]