组胺H_3受体拮抗剂对缺血缺氧性脑病新生大鼠神经保护作用及其机制研究  

The study of Histamine 3 Receptor Antagonist on Treatment and mechanism to Rat Model with Neonatal Hypoxic-ischemic Encephalopathy

作　　者：贾飞勇[1] 郝云鹏[1] 姜慧轶[1] 杜琳[1] 单玲[1] 王江涛[1] 

机构地区：[1]吉林大学白求恩第一医院儿内二科,吉林长春130021

出　　处：《中国实验诊断学》2011年第6期954-956,共3页Chinese Journal of Laboratory Diagnosis

基　　金：吉林省科技厅科研课题(编号:200905134)

摘　　要：目的探讨组胺H3受体拮抗剂硫丙咪胺对缺血缺氧性脑病新生大鼠的保护作用及其机制。方法将生后7天Wistar大鼠分为正常对照组、缺血缺氧脑病(HIE)模型组、治疗组;HIE模型组和治疗组应用左侧颈动脉结扎后缺氧方法制备HIE大鼠,治疗组进一步分为H3治疗组、H3+H1治疗组和H3+H2治疗组。H3治疗组给予5 mg/kg Thio腹腔注射;H2组先给予Dhp 5 mg/kgip,30 min后ip Thio 5 mg/kg;H1组先给予Cim100 mg/kgip,30 min后ip Thio 5 mg/kg;模型组和正常对照组给予生理盐水腹腔注射。并于注射完毕后6 h、24 h和72 h留取脑组织标本,对脑组织含水量、海马区组胺、MDA、SOD的含量进行检测。结果在给药后6 h、24 h和72 h的检测时间内,各组6 h与24 h脑组织含水量、MDA结果比较明显增高(P<0.05),SOD、海马区组胺结果比较明显减少(P<0.05);H3+H2组与模型组比较,脑组织含水量、MDA明显减少(P<0.05),SOD、海马区组胺明显增加(P<0.05);H3+H1组与H3组比较中,脑组织含水量、MDA明显增高(P<0.05),SOD、海马区组胺明显减少(P<0.05)。结论在颈动脉结扎后24 h,神经元损伤达到高峰。组胺H3受体拮抗剂对新生大鼠缺血缺氧性脑病神经元损伤具有保护作用。组胺H3受体拮抗剂对新生大鼠缺血缺氧性脑病神经元损伤的保护作用主要是通过组胺H2受体来实现的。Objective the study on H3 receptor antagonist compound to the neonatal with Hypoxia ischemia brain damage animal model therapeutic action mechanism research.By the measurement of brain organization hippocampal area histamine,MDA,SOD.Methods 7-day old neonatal Wistar rats was divided into 3group：normal comparison,HIE model groupand theraputic group.The theraputic group is devided into H3,H3＋H1,H3＋H2 Group.Rats in H3 group are intraperitoneally injected by 5 mg/kg Thio;Rats in H2 group are intraperitoneally injected by 5 mg/kg Dhp,and intraperitoneally injected by 5 mg/kg Thio 30 miniutes later;Rats in H1 group are intraperitoneally injected by 100 mg/kg Cim,and intraperitoneally injected by 5 mg/kg Thio 30miniutes later.Rats in normal comparison and HIE model group are intraperitoneally injected by Saline.6 h、24 h and 72 h after injection,get Brain tissue specimens to detect the value of Brain water content,hippocampal area histamine,MDA,SOD.Results 6,24 and72 hour after the therapy,we find,the comparison between the result of 24 h and 6h in each group shows that the Brain water content,MDA are both increased（P0.05）,while the hippocampal area histamine and SOD are both decreased（P0.05）.The Brain water content,MDA in each Thio group are significantly less than in the NS group（P0.05）,while the hippocampal area histamine and SOD are significantly more（P0.05）.The Brain water content,MDA in each Thio group are significantly nore than in the NS group（P0.05）,while the hippocampal area histamine and SOD are significantly less（P0.05）.Conclusion The histamine H3 receptor antagonist compound has the protective function for the neonatal with Hypoxia ischemia neuron damage.The histamine H3 receptor antagonist compound the protective function which damages to the neonatal rats with Hypoxia ischemia neuron damage is mainly realizes through the histamine H2 acceptor.

关 键 词：新生儿缺血缺氧性脑病 组胺 H3受体拮抗剂 H2受体拮抗剂 H1受体拮抗剂 

分 类 号：R722.1[医药卫生—儿科]