冻融法提取植物血凝素的研究  

Study on Extraction of PHA by Freeze-thaw Method

作　　者：刘艳艳[1] 秦光[1] 李继昌[1] 金希文[1] 

机构地区：[1]东北农业大学动物医学学院,黑龙江哈尔滨150030

出　　处：《黑龙江医药》2011年第3期357-359,共3页Heilongjiang Medicine journal

基　　金：黑龙江省教育厅振兴东北老工业基地建设重大项目(1152gzd07)

摘　　要：目的:采用不同缓冲液,确定冻融法提取植物血凝素的可行性。方法:在生理盐水、PBS、Tris、冰醋酸的不同缓冲液中,以吸收值、提取率和血凝效价为指标,比较传统浸提法与冻融法的差异。结果:表明采用PBS缓冲液的冻融法提取效果最佳(P<0.05),提取物的蛋白含量为312.33 mg/g,提取率为7.25%,血凝效价为7。结论:认为冻融法提取PHA法可行,并以PBS缓冲液最好。Objective：The feasibility is confirmated that PHA were extracted by freeze-thaw with differents buffer solution.Methods：The difference were compared with traditional extraction method and freeze-thaw method base on the indexes of absorption value 、extraction efficiency and hemagglutination titer in normal saline 、PBS、Tris and glacial acetic acid buffer solution.Results：The results showed that the freeze-thaw extraction was the best method with PBS buffer（P 0.05）,the protein content was 312.33 mg/g,extraction efficiency was 7.25%,hemagglutination titer was 7.Conclusion：It is feasible adoping freeze-thaw method to extract PHA,and the PBS buffer is the best method.

关 键 词：植物血凝素 冻融法 血凝效价 提取方法 

分 类 号：R853.7[医药卫生—航空、航天与航海医学] R282.71[医药卫生—临床医学]