乌头碱在大鼠体内的毒代动力学研究  

Study on Toxicokinetics of aconitine in SD rats

作　　者：陶长戈[1] 李文军[1] 彭成[1] 

机构地区：[1]成都中医药大学药学院,四川成都610075

出　　处：《湖北中医学院学报》2011年第3期21-23,共3页Journal of Hubei College of Traditional Chinese Medicine

基　　金：国家自然科学基金重点项目(30230410);国家重点基础研究发展计划(973计划)(2006CB5604705)

摘　　要：目的研究静脉注射给药后乌头碱在大鼠体内的毒动学过程。方法采用甲醇沉淀冻干法处理血浆样品,运用高灵敏的HPLC-MS-MS检测样品中乌头碱的含量。结果乌头碱分别在2.7-27ng/mL和27-81ng/mL范围内成良好的线性关系。最低检测限(LOD)为1.35ng/mL,S/N=6;定量限(LOQ)为2.7ng/mL,S/N=10。样品的回收率在93-97%,日内和日间RSD均小于5%。乌头碱在大鼠体内的过程符合二室模型,主要药动学参数为:AUC=(18581.8±469.6)ng/mL×min,CL=(0.001816±0.00078)mL/min,T1/2α=(0.432±1.28)min,T1/2β=(403.32±90.48)min,V(c)=0.086901±0.0018(μg/kg)/(ng/mL)。结论乌头碱在SD大鼠体内过程符合静脉注射二室模型。Objective To study aconitine toxicokinetics in SD rats.Methods Plasma samples by methanol precipitation freeze-dried was prepared,and detected by HPLC-MS/MS.Results Good linearity was obtained in the range of 2.7-27ng/mL and 27-81ng/mL.The sample recovery rate was 93%-97%;Both the inter-day RSD and intra-day RSD were less than 5%.The concentration-time curve of aconitine was consistent with the two-compartment model with iv.Injection.The main toxicokinetics parameters： AUC=（18581.8±469.6）（ng/mL）·min,CL=（0.001816±0.00078） mL/min,T1/2α=（0.432±1.28）min,T1/2β=（403.32±90.48）min,V（c）=0.086901±0.0018（μg/kg）/（ng/mL）.Conclusion The toxicokinetics model is two-compartment model after iv aconitine in SD rats.

关 键 词：乌头碱 液质联用 毒代动力学 

分 类 号：R285.5[医药卫生—中药学]