尾穗苋凝集素(ACA)基因植物表达载体的构建  

Construction of Plant Expression Vector of Amaranthus caudatus Agglutinin Gene(ACA)

作　　者：田华英[1] 庞彩红 夏阳 包颖[1] 咸洋 李双云 李丽 刘翠兰 毛秀红 燕丽萍 

机构地区：[1]曲阜师范大学生命科学学院,山东曲阜273165 [2]山东省林木遗传改良重点实验室/山东省林业科学院,山东济南250014

出　　处：《山东农业科学》2011年第6期10-13,共4页Shandong Agricultural Sciences

基　　金：济南市高校院所自主创新计划"优良城市园林绿化树种香樟转基因抗寒新种质选育";山东省农业良种工程"国槐;香樟耐盐抗旱抗寒转基因新品种选育"课题资助

摘　　要：尾穗苋凝集素(Amaranthus caudatusagglutin in,ACA)是一种存在于尾穗苋种子中的贮藏蛋白,属苋菜凝集素家族,对同翅目害虫如蚜虫、褐飞虱、叶蝉等有明显的致死作用。植物表达载体pCAMB IA2300-35S-OCS带有35S启动子及终止序列、卡那霉素(Kan)抗性基因NPTⅡ。载体pGEMT-ACA含有ACA基因,通过PCR扩增出ACA基因。把植物表达载体pCAMB IA2300-35S-OCS和扩增后的ACA基因用KpnⅠ和SalⅠ双酶切后经连接酶连接,得到植物表达载体pCAMB IA2300-ACA。将该载体分别导入农杆菌GV3101和LBA4404进行保存。Amaranthus caudatus agglutinin（ACA）,a kind of storage protein existed in the seeds of Amaranthus caudatus,belonged to amaranth lectin family and had lethal effect to homopteran pests such as aphid,Nilaparvata lugens and leafhopper.The vector pCAMBIA2300-35S-OCS contained the 35S promoter and its termination sequence,kanamycin（Kan） resistant gene NPT Ⅱ,and the vector pGEMT-ACA contained the ACA gene.The ACA gene was amplified by polymerase chain reaction（PCR） from the vector.After cleavage by KpnⅠand SalⅠand linkage by ligase,the plant expression vector pCAMBIA2300-ACA was constructed.Then the vector was transformed into the Agrobactrium tumefaciens GV3101 and LBA4404 for preservation.

关 键 词：ACA基因 植物表达载体 构建 

分 类 号：Q782[生物学—分子生物学]