转鲑鱼降钙素基因酵母对体外培养破骨细胞的影响  

Effect of Recombinant Saccharomyces cerevisiae Expressing Salmon Calcitonin on Osteoclast Culture in vitro

作　　者：姜勇 张辉[2] 王鹏[3] 赵喜喜 徐科凤 王健 张学成[4] 

机构地区：[1]青岛国家海洋科学研究中心,山东青岛266071 [2]中国农村技术开发中心,北京100045 [3]山东省农业科学院,山东济南250100 [4]中国海洋大学,山东青岛266003

出　　处：《山东农业科学》2011年第6期99-103,共5页Shandong Agricultural Sciences

基　　金：国家"863"高技术发展与研究计划项目(819-04-10)资助

摘　　要：用机械分离的方法获得新生Wistar大鼠的破骨细胞,在培养液中分别加入经肠激酶酶切的转鲑鱼降钙素基因酵母的上清液和密钙息(Miacalcic,鲑鱼降钙素注射液),以HE染色观察破骨细胞形态,Image-Pro Plus图像软件分析骨片上骨吸收陷窝的面积,骨片培养7天,吸收陷窝结果显示,转鲑鱼降钙素对破骨细胞吸收功能具有明显的抑制作用。The osteoclasts of new born Wistar mice were obtained by mechanical separation.The recombinant Saccharomyces cerevisiae expressing salmon calcitonin and restricted by enterokinase and Miacalcic were added into the culture solution respectively.The osteoclasts were observed by HE staining.After cultured for seven days,the area of bone resorption pits was analyzed by Image-Pro Plus image software.The results showed that the recombinant Saccharomyces cerevisiae expressing salmon calcitonin had obvious inhibitory action to the absorption function of osteoclasts.

关 键 词：鲑鱼降钙素 破骨细胞 细胞培养 

分 类 号：R322.71[医药卫生—人体解剖和组织胚胎学]