TACE对肝细胞癌VEGF、P16表达的影响  

The expression of VEGF and P16 in hepatocellular carcinoma after TACE

作　　者：李陆鹏[1] 张晶岩[1] 于友涛[1] 何东风[1] 

机构地区：[1]哈尔滨医科大学附属第三医院介入科,哈尔滨150081

出　　处：《实用肿瘤学杂志》2011年第3期228-232,共5页Practical Oncology Journal

基　　金：黑龙江省自然科学基金项目（D2005-53）

摘　　要：目的通过对比观察肝细胞癌经导管肝动脉化疗栓塞术（TACE）与单纯外科手术切除术后肿瘤组织中血管内皮生长因子（VEGF）、抑癌基因P16表达的变化，探讨TACE对肝细胞癌远期预后的影响。方法随机选取经病理证实的肝细胞癌手术所取得的标本石蜡组织包埋块65例。其中TACE术后手术切除的肝细胞癌组织标本35份（TACE组），未经任何治疗直接手术切除的肝细胞癌组织标本30份（对照组）。采用免疫组化技术检测术后肿瘤组织内VEGF和P16的表达水平，并进行比较分析。结果TACE组VEGF蛋白和P16蛋白阳性表达率均显著高于对照组。结论肝细胞癌TACE术后VEGF的高表达虽然可能会促进肿瘤的复发和转移，但TACE术后P16的高表达又会抑制VEGF作用的发挥，从而抑制肿瘤的复发和转移，两者的表达没有相关性。Objective To observe the expression differences of vascular endothelial growth factor （VEGF） and tumor suppressor gene P16 between the two tumor samples of hepatocellular carcinoma which has been separated respectively by transcatheter arterial chemoembolization （TACE）and surgical resection alone, and to study the effects of TACE on long - term prognosis of liver cancer. Methods The 65 paraffin embedded tissues blocks of hepatocellular carcinoma which have been pathologically confirmed were selected randomly. Among them ,the 35 specimens were got by transcatheter arterial chemoembolization（TACE group）, and the other 30 were separated by surgery without any treatment. The levels of VEGF and tumor suppressor gene P16 of the specimens were detected by immunohistochemical technique and comparative analysis. Results The expression of VEGF and P16 in TACE group was significantly higher than that of control group. Conclusion The high expression of VEGF of hepatocellular carcinoma after TACE may promote tumor recurrence and metastasis. However, the high expression of P16 may play a role in the inhibition of VEGF, thereby inhibiting tumor recurrence and metastasis, and there is no correlation between the expression of VEGF and P16.

关 键 词：肝细胞癌 经导管动脉化疗栓塞术 血管内皮生长因子 抑癌基因P16 

分 类 号：R815[医药卫生—放射医学]