甲基磺酸乙酯对烤烟种子发芽率的处理效应  

Mutagenic Effects of Ethyl Methane Sulfonate on Flue-cured Tobacco Seeds

作　　者：王军伟[1,2] 蒋彩虹[1] 宋志美[1] 张丽[1,2] 王鲁[1] 王元英[1] 刘贯山[1] 

机构地区：[1]中国农业科学院烟草研究所,农业部烟草类作物质量控制重点开放实验室,青岛266101 [2]中国农业科学院研究生院,北京100081

出　　处：《中国烟草科学》2011年第3期17-20,27,共5页Chinese Tobacco Science

基　　金：国家烟草专卖局项目“烟草突变体库创建与功能基因组学研究”(110200701022)

摘　　要：以不同浓度的甲基磺酸乙酯（EMS）作为化学诱变剂,烤烟不同品种的种子为供试材料,探讨了EMS化学诱变剂对烟草种子发芽率、根长等性状差异的诱变处理效应。结果表明,在浓度为0.1%~1.3%的处理范围内,EMS对种子的发芽率主要呈抑制作用,但低浓度处理对种子发芽率和种子根长有促进作用,高浓度处理对种子发芽率和种子根长均呈显著抑制作用;以半致死浓度为选择标准,确定了不同烤烟品种间EMS诱变处理种子的适宜浓度为0.35%~0.52%,致死浓度均为1.3%;供试品种间EMS处理反映的敏感性为云烟87=云烟97〉红花大金元〉中烟100〉K326。In this study,tobacco seeds of different varieties were treated with the chemical mutagen,ethyl methane sulfonate（EMS）.The germination rate and root lengths of the mutated seeds were measured.The results showed that germination was inhibited at EMS concentrations of 0.1%-1.3%,while the lower concentration EMS could increase the germination rate;the higher concentration EMS could significantly inhibit the germination and root elongation.By the selection criteria of lethal concentration 50,the appropriate concentrations of EMS for treating flue-cured tobacco seeds were 0.35%-0.52% and lethal concentrations for all five varieties were 1.3%.The sensitivity of tested tobacco varieties to EMS in descending order was Yunyan87=Yunyan97 Honghuadajinyuan Zhongyan100 K326.

关 键 词：烤烟 种子 EMS 半致死浓度 突变体 

分 类 号：S572[农业科学—烟草工业]