利用ISSR分析贵州部分烟草品种(系)的遗传关系  

Genetic Relatives Analysis of Guizhou Local Tobacco Germplasms Based on Inter-simple Sequence Repeat(ISSR)

作　　者：胡重怡[1] 蔡刘体[1] 

机构地区：[1]贵州省烟草科学研究所,贵阳550018

出　　处：《中国烟草科学》2011年第3期28-31,共4页Chinese Tobacco Science

基　　金：贵州省烟草专卖局项目(黔烟科2007-03)

摘　　要：利用ISSR标记,对12个贵州地方品种（系）和5个贵州主要种植品种分析亲缘关系。结果显示,有23条ISSR引物在烟草上能扩出清晰条带,其中9条引物能扩增出明显多态,能把17个品种（系）区分开。利用NTSYS 2.0软件对多态条带进行统计;用SimQual计算遗传相似系数,遗传相似系数在0.2127~0.7872之间变动。根据UPGMA进行聚类分析结果表明,17个品种（系）可以分为3个组群。ISSR marker was used to find genetic relationship between 12 local tobacco varieties（strains） and 5 major tobacco varieties of Guizhou in this study.The result showed that 23 ISSR primers tested could produce distinct fingerprints among 17 varieties（strains） of tobacco.An acceptable DNA polymorphic fingerprints was constructed with 9 primers which could be used to identify these tobacco varieties（strains）,all varieties（strains） were distinguished from each other and showed genetic diversity.The result from the analysis were used by NTSYS 2.0.The result showed that genetic similarity of these cultivated varieties（strains） from 0.212 7 to 0.787 2 by SimQual and these 17 varieties（strains） of tobacco could be divided into three groups by UPGMA clustering analysis.

关 键 词：ISSR 烟草 亲缘关系 

分 类 号：TS413[农业科学—烟草工业]