妊娠母鼠静脉注射葡萄球菌肠毒素B对胎鼠胸腺发育的影响  

Effect of maternal infection by staphylococcal enterotoxin B during pregnancy on the development of fetal thymus

作　　者：刘从森[1] 刘婷婷[1] 孔晓明[1] 朱翔[1] 刘勇[1] 闵宏林[1] 管俊昌[1] 

机构地区：[1]蚌埠医学院病原生物学教研室安徽省感染与免疫重点实验室,安徽蚌埠233030

出　　处：《蚌埠医学院学报》2011年第6期545-546,550,共3页Journal of Bengbu Medical College

基　　金：国家自然科学基金资助项目(81070506)

摘　　要：目的:观察妊娠母鼠静脉注射葡萄球菌肠毒素B(SEB)对胎鼠胸腺发育的影响。方法:将孕鼠随机分为实验组及对照组,每组12只;实验组和对照组孕鼠在妊娠第16天分别尾静脉注射SEB 15μg和等体积的生理盐水;于妊娠21天打开母鼠腹腔取出胎鼠,称量胎盘、胎鼠及其胸腺的湿重和干重;胎鼠胸腺细胞以抗CD4-APC及抗CD8-PE双标染色后用流式细胞仪检测T淋巴细胞各亚群。结果:妊娠母鼠静脉注射SEB后可明显减少胎鼠胸腺的湿重和干重(P<0.01),减少CD4-CD8+、CD4+CD8+T细胞(P<0.01和P<0.05),但明显增加CD4+CD8-T细胞(P<0.01);胎鼠和胎盘的干、湿重与对照组差异均无统计学意义(P>0.05)。结论:SEB可明显影响胎鼠胸腺的发育。Objective：To investigate the influence of maternal infection by staphylococcal enterotoxin B （SEB） during pregnancy on the development of fetal thymus. Methods：The pregnant rats at the gestational day（GD） 16 were randomly divided into two groups：the experimental group of 12 rats injected intravenously by 15 p,g SEB and the control group of 12 rats by vehicle. At GD 21 the fetuses of two groups were acquired after the pregnant rats were killed, then the weights of placenta, fetal thymus and the whole fetal body were measured. The percentages of CD4^＋ and CD8^＋ T cell subsets in thymus was detected by flow cytometry. Results ： Maternal infection by SEB during pregnancy decreased the weights of fetal thymus as well as the percentages of both CD4^- CD8^＋ and CD4^＋ CD8^＋ T cells, while the percentage of CD4^＋ CD8^- T cells was increased compared to that of the control group （P 〈 0.01 and P 〈 0.05）. But the weights of placenta and the whole fetal had no difference between the two groups（ P 〉 0.05）. Conclusions：The thymus development of fetus can be affected by maternal infection by SEB during pregnancy.

关 键 词：妊娠 胎鼠 胸腺发育 葡萄球菌肠毒素B 

分 类 号：R714.1[医药卫生—妇产科学]