鸭白细胞抗菌肽对小鼠免疫功能的影响  

Effects of Antimicrobial Peptides from Duck Leukocytes on Immunity of Mice

作　　者：耿娟[1] 王永才[1] 陈丽颖[1] 

机构地区：[1]河南农业大学牧医工程学院,河南郑州450002

出　　处：《河南农业科学》2011年第4期142-145,共4页Journal of Henan Agricultural Sciences

基　　金：河南省科技攻关项目(0523010500);河南省重点科技攻关项目(072102130020)

摘　　要：从樱桃谷鸭血液中提取出白细胞并制备粗提抗菌肽,分别以低、中、高3种剂量(质量浓度分别为1.5 g/L、7.5 g/L、15 g/L)灌胃清洁级昆明系小鼠,0.2 mL/(d.只),同时设0.01%醋酸溶液对照组和蛋白对照组(10 g/L牛血清白蛋白)。连续灌胃14 d后,测定小鼠的免疫器官指数、E玫瑰花环形成率、碳粒廓清指数;同时采用流式细胞术测定小鼠外周血T细胞亚群的水平。结果表明,各剂量的白细胞抗菌肽均能显著提高小鼠的E玫瑰花环形成率和CD3+水平;同时15 g/L的白细胞抗菌肽能明显提高小鼠的免疫器官指数、巨噬细胞吞噬指数、CD4+、CD4+/CD8+水平。初步说明鸭白细胞粗提抗菌肽能明显增强小鼠的免疫功能。Crude extractions of antimicrobial peptides from Yingtaogu duck blood was administrated in Kunming strain mice for two weeks through gavage at a daily dose of 0.2mL with the concentrations of 1.5g/L,7.5g/L or 15g/L,respectively.For negative controls,0.2mL of 0.01% acetic acid or 10g/L BSA was separately used.The serum phagocytic rate,phagocytic index,and weight of the immune organs（spleen and thymus） were measured,whilst the total number and subgroups of tlymphocytes were counted through flow cytometry（FCM）.The results showed that the extract improved the E-rosette ratio and the total number of CD3＋ T cells.Compared to other groups,the extract used at a dosage of 15mg/L significantly improved the immune organ weight index（P0.05）,serum phagocytic index（P0.01）,and the percentages of both CD4＋ and CD8＋ T cells（P0.01）.This indicates that the immunity of mice was significantly improved by antimicrobial peptides extracted from duck leukocytes.

关 键 词：鸭 白细胞抗菌肽 免疫 流式细胞术 

分 类 号：S834[农业科学—畜牧学]