百棉3号、百棉018杂交种及其亲本SSR指纹图谱构建  

Establishment of SSR Fingerprinting for Baimian 3,Baimian 018 Hybrids and Their Parents

作　　者：李成奇[1] 王晓芸[1] 刘莹[1] 王清连[1] 

机构地区：[1]河南科技学院河南省高等学校作物分子育种重点开放实验室/棉花研究所,河南新乡453003

出　　处：《河南农业科学》2011年第6期56-59,共4页Journal of Henan Agricultural Sciences

基　　金：国家转基因生物新品种培育科技重大专项(2008ZX08005-001;2008ZX08005-002);国家农业科技成果转化资金项目(2010276);河南省重点科技攻关计划项目(092102110025);河南省自然科学研究计划项目(2010A210006)

摘　　要：以我国不同生态棉区种植的33份棉花自交系品种和1份棉花杂交种品种为背景材料,构建了百棉3号、百棉018杂交种及其亲本的SSR指纹图谱。20对核心引物分属棉花15条染色体,共检测到116个等位基因,平均5.8个;多态信息含量(PIC)值平均为0.723。引物NAU2627、NAU1255、NAU1043、NAU1085在百棉3号亲本间的多态性为共显;引物NAU1028、NAU2092、NAU3337、NAU4024、NAU1043、NAU3110在百棉018亲本间的多态性为共显。引物组合NAU1028/NAU1085、NAU1028/NAU1043/NAU5262、DPL0209/NAU2869、NAU1028/NAU5104、NAU2121/NAU3703、NAU2092/NAU2869可分别将百棉3号母本、百棉3号父本、百棉3号、百棉018母本、百棉018父本和百棉018与其他所有材料区分开。2个杂交种的共显性分子标记和引物组合指纹图谱相结合,为其真伪鉴定和纯度检测提供了分子依据。SSR markers were used to analyze DNA fingerprinting of Baimian 3,Baimian 018 hybrids and their parents with background materials including 33 cotton inbred varieties and 1 hybrid variety,which were once planted or were still being planted in different ecological cotton zones.20 SSR core primers belonged to 15 chromosomes and 116 alleles were detected by them.The average alleles and polymorphic information content of 20 SSR primers were 5.8 and 0.723,respectively.NAU2627,NAU1255,NAU1043,NAU1085 were codominant markers between Baimian 3 parents,and NAU1028,NAU2092,NAU3337,NAU4024,NAU1043,NAU3110 were codominant markers between Baimian 018 parents.Primer combinations NAU1028/NAU1085,NAU1028/NAU1043/NAU5262,DPL0209/NAU2869,NAU1028/NAU5104,NAU2121/NAU3703 and NAU2092/NAU2869 could be used to identify Baimian 3 female parent,Baimian 3 male parent,Baimian 3,Baimian 018 female parent,Baimian 018 male parent,Baimian 018,respectively.The fingerprinting of both codominant molecular markers and primer combinations of 2 hybrids provided the molecular basis for their truth appraisal and purity examination.

关 键 词：棉花 杂交种 亲本 简单序列重复标记 指纹图谱 

分 类 号：S562[农业科学—作物学]