柯里拉京对血吸虫病小鼠肝组织白细胞介素-13表达的影响及其抗肝纤维化疗效研究  

Effects of Corilagin on expression of IL-13 in schistosomiasis liver fibrosis of mice liver tissue and therapeutic effect of anti-fibrosis

作　　者：黄艺芬[1] 陈盛铎[2] 王月凤[3] 史华新[1] 金怀海[1] 张莹[1] 赵雷[3] 

机构地区：[1]湖北中医药大学 [2]湖北省中医院 [3]华中科技大学同济医学院附属协和医院

出　　处：《中西医结合肝病杂志》2011年第3期156-158,I0014,共4页Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases

基　　金：国家自然科学基金资助项目(No.30901248)

摘　　要：目的:研究柯里拉京对感染日本血吸虫病所致小鼠肝组织虫卵肉芽肿的疗效和抗肝纤维化的作用,并观察其对肝组织白细胞介素13(IL-13)表达的影响。方法:21只BALB/c小鼠随机分为正常组、模型组、治疗组,每组7只,模型组和治疗组小鼠同时感染日本血吸虫,感染6周后治疗组小鼠给予柯里拉京生理盐水溶液灌胃30天,同时正常组和模型组小鼠给予等量生理盐水灌胃。干预结束后比较各组小鼠肝脏虫卵肉芽肿大小、纤维化评分,同时检测肝组织羟脯氨酸与IL-13含量。结果:柯里拉京组小鼠肝脏虫卵肉芽肿大小、纤维化评分、肝组织羟脯氨酸及IL-13含量明显低于模型组,均具有统计学意义(P<0.05或P<0.01)。结论:柯里拉京能明显减轻血吸虫虫卵肉芽肿所致肝纤维化,减少促纤维化型细胞因子IL-13的分泌,延缓纤维化的进展。Objective：To observe the effects of Corilagin on S.japonicum-induced granulomas and fibrosis and to investigate the influences on IL-13 expression in infected mice.Methods：Twenty and one balb/c mice were randomly divided into 3 groups,normal group,model group and treated group,7 mice per group,all animals for each experiment were infected on the same day except for the normal group.In treated group,the Corilagin was administrated 6 weeks post infection,physiological saline was administrated the other groups（30 days of successive administration）.To compare the size of egg granulomas,fibrosis scores,the concentration of hydroxyproline and IL-13 in liver tissue after treated.Results：Upon the examination of Corilagin-treated mice,the size of egg granuloma,fibrosis scores,the concentration of hydroxyproline and IL-13 in liver tissue were significantly reduced compared with the model mice.Conclusion：Corilagin might alleviate the hepatic granulomas and fibrosis caused by S.japonicum and these effect of Corilagin might be associated with its ability to decrease the level of profibrotic cytokines IL-13.

关 键 词：血吸虫病 肝纤维化 柯里拉京/药效学 白细胞介素-13 

分 类 号：R532.21[医药卫生—内科学]