线叶嵩草内生细菌的鉴定及溶磷效果的初步研究  

Identification of an endophytic phosphate-solubilizing bacteria isolated from Kobresia capillifolia

作　　者：王辰月[1] 陈秀蓉[1] 杨成德[1] 叶震[1] 李振东[1,2] 

机构地区：[1]甘肃农业大学草业学院,草业生态系统教育部重点实验室,中-美草地畜牧业可持续发展研究中心,甘肃兰州730070 [2]甘肃省社会科学研究院,甘肃兰州730070

出　　处：《甘肃农业大学学报》2011年第3期99-103,108,共6页Journal of Gansu Agricultural University

基　　金：甘肃省自然科学基金(096RJZA003)

摘　　要：菌株LXA10分离于东祁连山高寒草地线叶嵩草(Kobresia capillifolia),经PKO培养基培养和溶磷活性测定.结果表明:菌株LXA10对无机磷的溶解能力为28.14 mg.L-1,溶磷量为5.60 mg.g-1.不同的培养基成分对菌株LXA10的溶磷能力有影响,培养基中Fe2+、Mn2+、Mg2+等离子可提高LXA10的溶磷能力,菌株LXA10对培养基pH没有显著影响.经16S rDNA序列分析,并结合形态特征及生理生化指标,鉴定菌株LXA10为枯草芽孢杆菌(Bacillus subtilis).Strain LXA10 was isolated from Kobresia capillifolia,a kind of advantageous plants in alpine grassland in the Eastern Qilian Mountains.The PKO culture results indicated that capacity of phosphate-solubilizing of endophytic bacteria LXA10 was 28.14 mg·L-1,and the capacity was obviously different in different culture media.Fe2＋、 Mn2＋ 、Mg2 could promoted the LXA10 strain phosphate solubilization ability.It was also found that the capacity was not directly related to pH of media.The strain was identified as Bacillus subtilis,based on its 16S rDNA sequence,physiological and biochemical characteristics.

关 键 词：溶磷 内生溶磷细菌 线叶嵩草 枯草孢杆菌 

分 类 号：Q93-33[生物学—微生物学]