从ITS序列探讨锦鸡儿属(Caragana Fabr.)植物系统关系  

Phylogenetic Relationships of Caragana (Fabaceae): Evidence from nrITS Sequences

作　　者：康红梅[1,2] 白娟[3] 陈康[4] 王刚[1] 

机构地区：[1]兰州大学生命科学院,甘肃兰州730000 [2]甘肃省科学院生物研究所,甘肃兰州730000 [3]西北农林科技大学生命科学院,陕西杨凌712100 [4]中国科学院海洋研究所,山东青岛266071

出　　处：《西南农业学报》2011年第3期1099-1103,共5页Southwest China Journal of Agricultural Sciences

基　　金：国家重点基础研究发展规划(2002CB111505)

摘　　要：以锦鸡儿属11个系29种为代表材料,选择性扩增nrITS序列并双向测序,结合黄耆亚族Astralinae （Adens） Benth其他6属7个代表种的nrITS序列进行最大简约性（MP）和最小进化（ME）的系统发育分析。结果显示：锦鸡儿植物ITS序列长度在611~614 bp之间,与外类群排序后长度为655 bp,共有170个可变位点,其中107个简约信息位点,简约信息位点在总排序序列中达16.3%,可以为属内及属间系统关系提供有力的分子证据;锦鸡儿属在系统发育上不是一个单系类群,与丽豆属（Calophaca Fish. ex DC.）植物具有极为相近的亲缘关系;Sect.tragacanthoides的种在MP和ME进化树中位置分散,其组的分类有待进一步研究。卷叶锦鸡儿（C. ordosica,新种）虽然形态上与垫状锦鸡儿（C. tibetica）相似,但它与荒漠锦鸡儿（C. roborovskyi）遗传学关系紧密;C. davazamcii是一个独立的种。Internal transcribed spacer（ITS） sequences of nuclear ribosomal DNA（nrDNA） from 29 taxa of Caragana species and seven close relatives（all belong to Astralinae（Adens） Benth） were used for phylogenetic analysis.The results were shown that： Length of the entire ITS region ranges from 611 to 614bp in Caragana species.The aligned sequences nrITS of Caragana are 655bp,and 170 sites are variable,with 107 phylogenetically informative sites.The phylogenetically informative sites are 16.3 % of the total aligned sequences.The ITS sequences data are useful to resolve some relationships at lower taxonomic levels within Caragana.The Caragana Fabr.is not a monophyletic group with very close connection with Calophaca tianschanica.The ITS data reveal that the species of Sect.tragacanthoides were dispersed in MP tree or ME tree.Although the morphology of C.ordosica is similar to C.tibetica,the ITS results revealed an unexpectedly close relativeship to C.roborovskyi.The ITS data also indicated C.davazamcii,C.korshinskii,C.intermedia,and C.microphylla are different species.

关 键 词：锦鸡儿 ITS 系统关系 

分 类 号：Q949.751.9[生物学—植物学]