转Bt基因玉米对土壤微生物量碳氮的影响  

Effects of transgenic Bt maize on soil microbial biomass carbon and nitrogen

作　　者：刘玲[1,2] 赵建宁[2] 王生荣[1] 杨殿林[2] 李刚[2] 娜布其[2,3] 娜日苏[2,3] 

机构地区：[1]甘肃农业大学草业学院/草业生态系统教育部重点实验室/中-美草地畜牧业可持续发展研究中心/甘肃省草业工程实验室,甘肃兰州730070 [2]农业部环境保护科研监测所/农业部转基因生物生态环境安全监督检验测试中心,天津300191 [3]内蒙古师范大学生命科学与技术学院,内蒙古呼和浩特010022

出　　处：《草原与草坪》2011年第3期35-39,共5页Grassland and Turf

基　　金：转基因生物新品种培育重大专项(2011ZX08011-002;2011ZX08012-005);中央级公益性科研院所基本科研业务费专项资金项目(农业部环境保护科研监测所)资助

摘　　要：利用三室根箱装置获得玉米生长室(S1)、根际土(S2)、非根际土(S3)3个不同根区土壤,研究了转基因玉米Bt11及其非转基因亲本玉米播种后40,50和60 d各根区土壤微生物量碳、氮的变化。结果表明,转Bt基因玉米播种后40 d各根区土壤微生物量碳与非转基因亲本玉米相比无显著差异,土壤微生物量氮显著升高;播种后50 d土壤微生物量碳、氮均显著高于亲本;播种后60 d转Bt基因玉米S1根区土壤微生物量碳、氮含量显著降低,S2根区土壤微生物量碳较亲本玉米显著下降,微生物量氮则显著升高,S3根区土壤微生物量碳、氮均高于亲本玉米。转Bt基因玉米土壤微生物量碳氮比在各采样时期均与亲本存在差异,依玉米生长时期和根区的变化而不同。Three-room rhizobox method were used to collect soil samples from three different root zones of maize growth room（S1）,rhizosphere（S2） and non-rhizosphere（S3） respectively.The changes of content of soil microbial biomass C and N at 40 d、50 d and 60 d after sowing transgenic Bt maize and its parental maize without transgenic were studied.The results showed that the difference of soil microbial biomass C in three different root zones between transgenic Bt maize and non transgenic maize was not significant at 40 days,but the difference of microbial biomass N wais significant.The difference of soil microbial biomass C and N of Bt maize and non transgenic maize were significant at 50 d.At 60 days,the soil microbial biomass C and N of Bt maize significantly decreased in S1 root zone,the soil microbial biomass C decreased and soil microbial biomass N increased in S2 root zone of Bt maize,both of soil microbial biomass C and N in S3 root zone of Bt maize were higher than non transgenic maize.The ratio of soil microbial biomass C/N was significant difference between transgenic Bt maize and the parental maize and it changed according to root zones and growth period.

关 键 词：转BT基因玉米 土壤微生物量碳氮 根箱 

分 类 号：S154[农业科学—土壤学]