一株来自东祁连山高寒草地土壤纤维素分解真菌培养特性的研究  

Study on cultural characteristic of one cellulose-decomposing stains of fungus from alpine grassland soil in eastern Qilian Mountains

作　　者：芦光新[1,2] 刘雯[1] 卞静[1] 傅美涛[1] 陈秀蓉[1] 

机构地区：[1]甘肃农业大学草业学院/草业生态系统教育部重点实验室/中-美草地畜牧业可持续发展研究中心/甘肃省草业工程实验室,甘肃兰州730070 [2]青海大学农牧学院草业科学系,青海西宁810016

出　　处：《草原与草坪》2011年第3期50-55,共6页Grassland and Turf

基　　金：国家自然科学基金项目(30471232)资助

摘　　要：对一株来自东祁连山高寒草地土壤具有纤维素分解能力的真菌的培养特性进行了研究。结果表明:供试菌株的菌丝生长温度5～35℃,最适生长温度30℃;在参试的pH范围均能生长,并且在pH7.08的环境生长最快;供试菌株具有一定的耐盐和抗干旱胁迫的能力;对不同的碳、氮源利用能力不同,在供试的11种碳源和12种氮源中,乳糖和大豆蛋白胨分别为最适碳源和氮源。以1.5%的水琼脂培养基为对照菌株,在黑麦草(Lolium perenne)浸提液培养基上生长较快,三叶草(Trifolium repens)的浸提液培养基上的生长速度较慢,其余的天然纤维素浸提液培养基上的生长速度差异不明显。Cultural characteristic of one cellulose-decomposing stain of fungus from alpine grassland soil in eastern Qilian Mountains were studied.The results showed that this strains was able to grow at 5～35 ℃.The optimal cultural temperature and pH values for mycelial growth was 30 ℃ and 7.08 respectively.The strain has ability of salt and PEG stress tolerance.It varied in its viability on media containing different carbon and nitrogen nutrients.The preferential carbon and nitrogen source was lactose and soybean peptone,respectively.Compare to the 1.5% water agar medium,the growth of the stain was faster in ryegrass extract,but slower in clover extract.The growth of the mycelium was not significantly difference in medium with different natural cellulose extract.

关 键 词：高寒草地 纤维素分解真菌 培养特性 

分 类 号：S154.3[农业科学—土壤学]