愈疡胶囊对乳腺增生大鼠雌激素影响的实验研究  

The Experimental Study of Yuyang Capsule on the Estrogen in the Heperphasia of Mammary Gland Rats

作　　者：罗伦才 黄兰雅 季小平[1] 李列平[1] 邢晋苓 刘志康[1] 

机构地区：[1]四川省凉山彝族自治州第二人民医院,四川凉山615000 [2]雅安职业技术学院药学检验系,四川雅安625000

出　　处：《中国药业》2011年第13期5-6,共2页China Pharmaceuticals

基　　金：四川省中医药管理局项目;项目编号:2008-14

摘　　要：目的探讨愈疡胶囊对乳腺增生病的治疗作用及其机理。方法将成年雌性未孕大鼠随机分为空白对照组、模型对照组、阳性对照组和愈疡胶囊组,每组8只,除空白对照组外,采用苯甲酸雌二醇联合黄体酮肌肉注射方法复制大鼠乳腺增生模型并分别给药。采用酶联免疫法检测各组大鼠血清中孕酮含量和乳腺组织匀浆中雌二醇受体含量,采用HE染色法进行乳腺组织形态学观察与评价。结果与空白对照组比较,模型对照组大鼠血清孕酮含量明显下降(P<0.01),乳腺组织雌二醇受体含量明显升高(P<0.01),乳腺组织切片均有典型的乳腺增生;经愈疡胶囊治疗后,大鼠血清孕酮含量显著升高(P<0.05),乳腺组织雌二醇受体含量明显下降(P<0.01),乳腺组织切片中典型的乳腺增生情况得到明显改善。结论愈疡胶囊能明显抑制和治疗大鼠的乳腺增生,其作用方式可能是通过调节大鼠体内的激素分泌水平,减弱雌激素和孕激素在乳腺的生物学效应。Objective To investigate Yuyang Capsule in treating mammary glands and its mechanism.Methods Adult female virgin rats were randomly divided into control group,model control group,positive control group and Yuyang Capsule group,8 rats in each group.In addition to the control group,the use of progesterone,estradiol benzoate intramuscular injection method combined rat and animal models of breast cancer were administered,using enzyme-linked immunosorbent assay of serum progesterone levels to detect estradiol in breast tissue alcohol receptor content;by HE staining for histological examination and evaluation of breast.Results Compared with the control group,model control group,serum progesterone levels decreased significantly（P 0.01）,estrogen receptor content of breast tissue was significantly higher（P 0.01）,breast biopsy are typical hyperplasia;the Yuyang Capsule treatment,serum progesterone levels increased significantly（P 0.05）,estrogen receptor content of breast tissue decreased significantly（P 0.01）,breast biopsy in a typical situation has markedly improved breast cancer.Conclusion Yuyang Capsule can significantly inhibit the proliferation of rat mammary gland,and its mode of action may be by regulating the body＇s hormone levels in rats,diminished estrogen and progesterone in the breast of the biological effects.

关 键 词：愈疡胶囊 乳腺增生大鼠模型 孕酮 雌二醇受体 

分 类 号：R285.5[医药卫生—中药学] R271.44[医药卫生—中医学]