反相高效液相色谱法测定烧伤涂膜中黄芩苷含量  

Content Determination of Baicalin in Burn Membrane by RP-HPLC

作　　者：张秋红[1] 张忠义[2] 王曙东[1] 

机构地区：[1]中国人民解放军南京军区南京总医院制剂科,江苏南京210002 [2]南方医科大学珠江医院药剂科,广东广州510282

出　　处：《中国药业》2011年第13期26-27,共2页China Pharmaceuticals

摘　　要：目的建立测定烧伤涂膜中黄芩苷含量的方法。方法用薄层色谱法鉴别涂膜剂中的黄芩,用反相高效液相色谱法测定涂膜中黄芩苷含量。结果薄层色谱法鉴定结果满意;黄芩苷质量浓度在0.712～3.00 g/L范围内与峰面积线性关系良好,r=0.998 2(n=6),平均加样回收率为95.37%,RSD为1.55%(n=6)。结论定性定量方法简便、可靠、重现性好,可作为烧伤涂膜的质量控制方法。Objective To establish methods for determining the content of baicalin in Burn Membrane.Methods TLC method was applicated to determine Stellaria baicalensis Georgi.RP-HPLC method was used for detecting the content of baicalin in Burn Membrane.Results The components were determined well.Baicalin had a good linearity in the range of 0.712-3.00 g/L,r=0.998 2（n=6）,and the average recovery rate was 95.37%with RSD of 1.55%（n=6）.Conclusion The method is simple.accurate and reproducible and can be used for controlling the quality of burn membrane.

关 键 词：烧伤涂膜 黄芩 薄层色谱法 反相高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学] R286.0[医药卫生—中医学]