泡沫细胞寡核苷酸适配子PM2的特异性研究  

Research on the Specificity of PM2 Aptamer about THP-1 Macrophage Derived Foam Cells

作　　者：林广庆[1] 严鹏科[1] 汪江波[2] 张慧[3] 段才闻[3] 李世煌[3] 

机构地区：[1]广州医学院附属第三医院药剂科,广东广州510150 [2]广西壮族自治区南溪山医院药剂科,广西桂林541002 [3]南华大学药物药理研究所,湖南衡阳421001

出　　处：《中国医药科学》2011年第12期20-23,共4页China Medicine And Pharmacy

摘　　要：目的研究寡核苷酸适配子对动脉粥样硬化病变组织结合的特异性。方法将前期研究获得的特异性适配子命名为PM2,FITC标记后,荧光显微镜分别观察适配子与血管平滑肌细胞、巨噬细胞、内皮细胞、巨噬细胞源性泡沫细胞结合情况。采用高脂方法建立兔的动脉粥样硬化模型。利用PCR方法和荧光显微镜观察适配子与动脉粥样硬化病变结合情况。结果 PM2适配子与巨噬细胞源性泡沫细胞高度特异性结合,但不与血管平滑肌细胞、巨噬细胞、内皮细胞结合;PM2适配子能特异性结合动脉粥样硬化斑块组织,而不结合正常动脉组织结合。结论筛选出的PM2适配子特异性结合巨噬细胞源性泡沫细胞和动脉粥样硬化病变。Objective To observe the binding specificity of aptamers and THP-1 macrophage derived foam cells and atherosclerotic lesions. Methods The FITC-labeled No 40 aptamers （ PM2 ）, binding to THP-1 macrophages, vascular smooth cells, endothelial cells and THP-1 macrophage derived foam cells were observed by fluorescence microscopy respectivly.Model of atherosclerosis was formed by high-fat feeding rabbit methods.The binding of aptamers and atherosclerotic lesions were detected by fluorescence microscopy or PCR. Results The PM2 aptamers didn＇ t bind to THP-1 maerophages, vascular smooth cells and endothelial cells but bind to THP-1 macrophage derived foam cells and atherosclerotic lesions. Conclusion We have obtained PM2 aptamers specifically binding to THP-1 macrophage derived foam cells and atherosclerotic lesions.

关 键 词：SELEX 特异性 巨噬细胞源性泡沫细胞 动脉粥样硬化病变 

分 类 号：R543.5[医药卫生—心血管疾病]